Tiwari Priyanka, Ekal Neha S, Rudani Binny A, Bahadur Pratap, Tiwari Sanjay
Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER) Raebareli, Lucknow 226002, Uttar Pradesh, India.
Department of Chemistry, Veer Narmad South Gujarat University, Surat 395007, Gujarat, India.
Colloids Surf B Biointerfaces. 2023 May;225:113250. doi: 10.1016/j.colsurfb.2023.113250. Epub 2023 Mar 7.
This study examines the effect of surface oxygen groups upon ability of graphene oxide (GO) sheets in suppressing the fibrillation of lysozyme (LYZ). Graphite was oxidized using 6 and 8 wt equivalents of KMnO, and as produced sheets were abbreviated as GO-06 and GO-08, respectively. Particulate characteristics of sheets were characterized by light scattering and electron microscopic techniques, and their interaction with LYZ was analysed by circular dichroism (CD) spectroscopy. After ascertaining acid-driven conversion of LYZ to fibrillary form, we have shown that the fibrillation of dispersed protein can be prevented by adding GO sheets. Inhibitory effect could be attributed to binding of LYZ over the sheets via noncovalent forces. A comparison between GO-06 and GO-08 samples showed superior binding affinity of the latter. Higher aqueous dispersibility and density of oxygenated groups in GO-08 sheets would have facilitated the adsorption of protein molecules, thus making them unavailable for aggregation. Pre-treatment of GO sheets with Pluronic 103 (P103, a nonionic triblock copolymer), caused reduction in the adsorption of LYZ. P103 aggregates would have rendered the sheet surface unavailable for the adsorption of LYZ. Based on these observations, we conclude that fibrillation of LYZ can be prevented in association with graphene oxide sheets.
本研究考察了表面氧基团对氧化石墨烯（GO）片抑制溶菌酶（LYZ）纤维化能力的影响。使用6和8重量当量的KMnO对石墨进行氧化，所制备的片材分别简称为GO - 06和GO - 08。通过光散射和电子显微镜技术对片材的颗粒特性进行表征，并通过圆二色性（CD）光谱分析它们与LYZ的相互作用。在确定酸驱动LYZ转化为纤维状形式后，我们表明通过添加GO片可以防止分散蛋白质的纤维化。抑制作用可归因于LYZ通过非共价力与片材结合。GO - 06和GO - 08样品之间的比较表明后者具有更高的结合亲和力。GO - 08片中更高的水分散性和含氧基团密度会促进蛋白质分子的吸附，从而使其无法聚集。用普朗尼克103（P103，一种非离子三嵌段共聚物）对GO片进行预处理，导致LYZ的吸附减少。P103聚集体会使片材表面无法吸附LYZ。基于这些观察结果，我们得出结论，与氧化石墨烯片结合可以防止LYZ的纤维化。
