Faculty of Chemistry, Physicochemistry of Carbon Materials Research Group, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, Poland.
Department of Biochemistry, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland.
J Phys Chem B. 2022 Feb 3;126(4):928-933. doi: 10.1021/acs.jpcb.1c08294. Epub 2022 Jan 25.
The interaction between graphene oxide (GO) and lysozyme (LYZ) in aqueous solution was investigated for GO specific surface area determination and for the thermodynamic description of the process. It was experimentally proved that LYZ is a much better adsorbate than the most common methylene blue, allowing the determination of genuine GO surface area. Our fluorescence spectroscopy results indicate that LYZ molecules interact with GO at high- and low-affinity sites depending on the surface coverage, reflecting the protein mono- and multilayer formation, respectively. The lack of the secondary structure changes confirms LYZ usability as a model adsorbate. The calculated values of thermodynamic parameters (Δ(Δ) = -195.0 kJ/mol and Δ(Δ) = -621.3 J/molK) indicate that the interactions are exothermic, enthalpy-driven. All the reported results reveal the physical nature of the LYZ-GO interaction at the studied concentration ratios.
研究了氧化石墨烯（GO）与溶菌酶（LYZ）在水溶液中的相互作用，以确定 GO 的比表面积，并对该过程进行热力学描述。实验证明，LYZ 是一种比最常用的亚甲基蓝更好的吸附剂，能够确定真正的 GO 表面积。我们的荧光光谱结果表明，LYZ 分子根据表面覆盖率与 GO 在高亲和和低亲和位点相互作用，分别反映了蛋白质的单分子和多分子形成。缺乏二级结构变化证实了 LYZ 作为模型吸附剂的可用性。计算得到的热力学参数值（Δ(Δ) = -195.0 kJ/mol 和 Δ(Δ) = -621.3 J/molK）表明，相互作用是放热的，由焓驱动。所有报告的结果揭示了在研究的浓度比下 LYZ-GO 相互作用的物理性质。
