Wu Yike, Hao Mingyu, Li Weifeng, Xu Yun, Yan Dewen, Xu Yong, Liu Wenlan
The Department of Endocrinology, Shenzhen Second People's Hospital/the First Affiliated Hospital of Shenzhen University, Shenzhen Clinical Research Center for Metabolic Diseases, Shenzhen, China; The Center for Medical Genetics & Molecular Diagnosis, Shenzhen Second People's Hospital/the First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.
The Department of Endocrinology, Shenzhen Second People's Hospital/the First Affiliated Hospital of Shenzhen University, Shenzhen Clinical Research Center for Metabolic Diseases, Shenzhen, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Apr 1;1220:123662. doi: 10.1016/j.jchromb.2023.123662. Epub 2023 Mar 5.
Given the increasing morbidity of diabetes mellitus type 2 (T2DM) with peripheral neuropathy (PN), efficient screening for T2DM-PN is of great significance. Altered N-glycosylation is closely associated with T2DM progression, whereas its association with T2DM-PN remains uncharacterized. In this study, N-glycomic profiling was performed to identify the N-glycan features between T2DM patients with (n = 39, T2DM-PN) and without PN (n = 36, T2DM-C). Another independent set of T2DM patients (n = 29 for both T2DM-C and T2DM-PN) were utilized to validate these N-glycomic features. There were 10 N-glycans varied significantly between T2DM-C and T2DM-PN (p < 0.05 and 0.7 < AUC < 0.9), of which T2DM-PN was associated with increased oligomannose and core-fucosylation of sialylated glycans, and decreased bisected mono-sialylated glycan. Notably, these results were validated by an independent set of T2DM-C and T2DM-PN. This is the first profiling for N-glycan features in T2DM-PN patients, which reliably differentiates them from T2DM controls, thus providing a prospective profile of glyco-biomarkers for the screening and diagnosis of T2DM-PN.
鉴于2型糖尿病（T2DM）合并周围神经病变（PN）的发病率不断上升，对T2DM-PN进行有效的筛查具有重要意义。N-糖基化改变与T2DM的进展密切相关，但其与T2DM-PN的关系仍不明确。在本研究中，进行了N-糖组分析，以确定伴有PN的T2DM患者（n = 39，T2DM-PN）和不伴有PN的T2DM患者（n = 36，T2DM-C）之间的N-聚糖特征。另一组独立的T2DM患者（T2DM-C和T2DM-PN各n = 29）被用于验证这些N-糖组特征。T2DM-C和T2DM-PN之间有10种N-聚糖存在显著差异（p < 0.05且0.7 < AUC < 0.9），其中T2DM-PN与寡甘露糖增加、唾液酸化聚糖的核心岩藻糖基化增加以及二分单唾液酸化聚糖减少有关。值得注意的是，这些结果在另一组独立的T2DM-C和T2DM-PN中得到了验证。这是首次对T2DM-PN患者的N-聚糖特征进行分析，该分析能够可靠地将他们与T2DM对照组区分开来，从而为T2DM-PN的筛查和诊断提供了一种潜在的糖生物标志物分析方法。
