Department of Health Sciences and Technology, Institute of Food, Nutrition and Health, ETH Zurich, 8092 Zurich, Switzerland.
Department of Health Sciences and Technology, Institute of Food, Nutrition and Health, ETH Zurich, 8092 Zurich, Switzerland; Voevodsky Institute of Chemical Kinetics and Combustion, 630090 Novosibirsk, Russia.
Carbohydr Polym. 2023 Jun 1;309:120698. doi: 10.1016/j.carbpol.2023.120698. Epub 2023 Feb 13.
The interactions between dietary fibers (DFs) and small molecules are of great interest to food chemistry and nutrition science. However, the corresponding interaction mechanisms and structural rearrangements of DFs at the molecular level are still opaque due to the usually weak binding and the lack of appropriate techniques to determine details of conformational distributions in such weakly organized systems. By combining our previously established methodology on stochastic spin-labelling of DFs with the appropriately revised set of pulse electron paramagnetic resonance techniques, we present here a toolkit to determine the interactions between DFs and small molecules, using barley β-glucan as an example for neutral DF and a selection of food dye molecules as examples for small molecules. The proposed here methodology allowed us to observe subtle conformational changes of β-glucan by detecting multiple details of the local environment of the spin labels. Substantial variations of binding propensities were detected for different food dyes.
膳食纤维（DFs）与小分子之间的相互作用是食品化学和营养科学领域非常关注的问题。然而，由于通常较弱的结合以及缺乏适当的技术来确定此类组织松散系统中构象分布的细节，DFs 在分子水平上的相应相互作用机制和结构重排仍然不明确。通过将我们之前在 DFs 的随机自旋标记方面建立的方法与经过适当修改的脉冲电子顺磁共振技术集相结合，我们在此提出了一个工具包，以确定 DFs 与小分子之间的相互作用，使用大麦β-葡聚糖作为中性 DF 的示例和选择的食物染料分子作为小分子的示例。所提出的方法使我们能够通过检测自旋标记的局部环境的多个细节来观察β-葡聚糖的微妙构象变化。对于不同的食用染料，检测到了结合倾向的显著变化。
