从酸菜中分离出的HDC-01所产胞外多糖的纯化、表征及益生菌增殖效果
Purification, characterization and probiotic proliferation effect of exopolysaccharides produced by HDC-01 isolated from sauerkraut.
作者信息
Yu Liansheng, Ye Guangbin, Qi Xintong, Yang Yi, Zhou Bosen, Zhang Yunye, Du Renpeng, Ge Jingping, Ping Wenxiang
机构信息
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education and Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region and Key Laboratory of Microbiology, College of Heilongjiang Province and School of Life Sciences, Heilongjiang University, Harbin, China.
Hebei Key Laboratory of Agroecological Safety, Hebei University of Environmental Engineering, Qinhuangdao, China.
出版信息
Front Microbiol. 2023 Jun 27;14:1210302. doi: 10.3389/fmicb.2023.1210302. eCollection 2023.
In this study, an exopolysaccharide (EPS)-producing strain of HDC-01 was isolated from sauerkraut, and the structure, properties and biological activity of the studied EPS were assessed. The molecular weight of the isolated EPS is 2.505 × 10 Da. Fourier transform infrared spectrometry (FT-IR) and nuclear magnetic resonance (NMR) results showed that the EPS was composed of glucose/glucopyranose subunits linked by an -(1 → 6) glycosidic bond and contained an -(1 → 3) branching structure. X-ray diffraction (XRD) analysis revealed the amorphous nature of the EPS. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that the isolated EPS had a smooth and compact surface with several protrusions of varying lengths and irregularly shaped material. Moreover, the studied EPS showed good thermal stability, water holding capacity, and milk coagulation ability and promoted the growth of probiotics. EPS may be used as prebiotics in the fields of food and medicine.
在本研究中，从酸菜中分离出一株产胞外多糖（EPS）的菌株HDC-01，并对所研究的EPS的结构、性质和生物活性进行了评估。分离得到的EPS的分子量为2.505×10 Da。傅里叶变换红外光谱（FT-IR）和核磁共振（NMR）结果表明，该EPS由通过-(1→6)糖苷键连接的葡萄糖/吡喃葡萄糖亚基组成，并含有-(1→3)分支结构。X射线衍射（XRD）分析揭示了EPS的无定形性质。扫描电子显微镜（SEM）和原子力显微镜（AFM）显示，分离得到的EPS具有光滑致密的表面，有几个长度不同的突起和形状不规则的物质。此外，所研究的EPS表现出良好的热稳定性、持水能力和凝乳能力，并促进了益生菌的生长。EPS可在食品和医药领域用作益生元。
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