Davis R L, Weintraub H, Lassar A B
Department of Genetics, Hutchinson Cancer Research Center, Seattle, Washington 98104.
Cell. 1987 Dec 24;51(6):987-1000. doi: 10.1016/0092-8674(87)90585-x.
5-azacytidine treatment of mouse C3H10T1/2 embryonic fibroblasts converts them to myoblasts at a frequency suggesting alteration of one or only a few closely linked regulatory loci. Assuming such loci to be differentially expressed as poly(A)+ RNA in proliferating myoblasts, we prepared proliferating myoblast-specific, subtracted cDNA probes to screen a myocyte cDNA library. Based on a number of criteria, three cDNAs were selected and characterized. We show that expression of one of these cDNAs transfected into C3H10T1/2 fibroblasts, where it is not normally expressed, is sufficient to convert them to stable myoblasts. Myogenesis also occurs, but to a lesser extent, when this cDNA is expressed in a number of other cell lines. The major open reading frame encoded by this cDNA contains a short protein segment similar to a sequence present in the myc protein family.
用5-氮杂胞苷处理小鼠C3H10T1/2胚胎成纤维细胞，可将它们转化为成肌细胞，其频率表明一个或仅几个紧密连锁的调控基因座发生了改变。假设这些基因座在增殖的成肌细胞中作为多聚腺苷酸加尾（poly(A)+）RNA差异表达，我们制备了增殖的成肌细胞特异性扣除cDNA探针，以筛选成肌细胞cDNA文库。基于多个标准，选择并鉴定了三个cDNA。我们表明，将这些cDNA之一转染到通常不表达的C3H10T1/2成纤维细胞中，其表达足以将它们转化为稳定的成肌细胞。当该cDNA在许多其他细胞系中表达时，也会发生肌生成，但程度较小。该cDNA编码的主要开放阅读框包含一个短蛋白片段，类似于myc蛋白家族中存在的一个序列。
