牛血清白蛋白对Fe-NTA体外诱导细胞损伤的增强作用。

Enhancing effects of bovine serum albumin on cell injury in vitro induced with Fe-NTA.

作者信息

Yamada M, Okigaki T, Awai M

机构信息

Division of Cell Biology, Shigei Medical Research Institute, Okayama, Japan.

出版信息

Cell Biol Int Rep. 1987 Oct;11(10):707-16. doi: 10.1016/0309-1651(87)90129-9.

DOI:10.1016/0309-1651(87)90129-9

PMID:3690679

Abstract

Direct effects of ferric nitrilotriacetate (Fe-NTA) on normal rat liver epithelial cells (RL34) in serum-free culture were studied. More than 10 micrograms/ml iron of Fe-NTA was cytotoxic and the cytotoxicity was prevented by adding apotransferrin into culture medium. Also, bovine serum albumin (BSA), which is known to bind Fe-NTA, was found to promote the cytotoxicity, while fatty acids-free BSA (F-BSA) prevented it. This result indicates that fatty acids rather than albumin promote Fe-NTA cytotoxicity.

摘要

研究了次氮基三乙酸铁（Fe-NTA）对无血清培养的正常大鼠肝上皮细胞（RL34）的直接作用。Fe-NTA中超过10微克/毫升的铁具有细胞毒性，向培养基中添加脱铁转铁蛋白可防止这种细胞毒性。此外，已知能结合Fe-NTA的牛血清白蛋白（BSA）可促进细胞毒性，而无脂肪酸的牛血清白蛋白（F-BSA）则可防止细胞毒性。该结果表明，促进Fe-NTA细胞毒性的是脂肪酸而非白蛋白。