Welden Melanie, Poghossian Arshak, Vahidpour Farnoosh, Wendlandt Tim, Keusgen Michael, Schöning Michael J
Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, 52428 Jülich, Germany; Institute of Pharmaceutical Chemistry, Philipps University Marburg, 35032 Marburg, Germany.
MicroNanoBio, 40479 Düsseldorf, Germany.
Bioelectrochemistry. 2023 Jun;151:108397. doi: 10.1016/j.bioelechem.2023.108397. Epub 2023 Feb 12.
This work presents a new approach for the development of field-effect biosensors based on an electrolyte-insulator-semiconductor capacitor (EISCAP) modified with a stacked bilayer of weak polyelectrolyte and tobacco mosaic virus (TMV) particles as enzyme nanocarriers. With the aim to increase the surface density of virus particles and thus, to achieve a dense immobilization of enzymes, the negatively charged TMV particles were loaded onto the EISCAP surface modified with a positively charged poly(allylamine hydrochloride) (PAH) layer. The PAH/TMV bilayer was prepared on the TaO-gate surface by means of layer-by-layer technique. The bare and differently modified EISCAP surfaces were physically characterized by fluorescence microscopy, zeta-potential measurements, atomic force microscopy and scanning electron microscopy. Transmission electron microscopy was used to scrutinize the PAH effect on TMV adsorption in a second system. Finally, a highly sensitive TMV-assisted EISCAP antibiotics biosensor was realized by immobilizing the enzyme penicillinase onto the TMV surface. This PAH/TMV bilayer-modified EISCAP biosensor was electrochemically characterized in solutions with different penicillin concentrations via capacitance-voltage and constant-capacitance methods. The biosensor possessed a mean penicillin sensitivity of 113 mV/dec in a concentration range from 0.1 mM to 5 mM.
这项工作提出了一种开发场效应生物传感器的新方法，该传感器基于用弱聚电解质和烟草花叶病毒（TMV）颗粒的堆叠双层作为酶纳米载体修饰的电解质-绝缘体-半导体电容器（EISCAP）。为了提高病毒颗粒的表面密度，从而实现酶的密集固定，将带负电荷的TMV颗粒加载到用带正电荷的聚（烯丙胺盐酸盐）（PAH）层修饰的EISCAP表面上。通过逐层技术在TaO栅极表面制备PAH/TMV双层。通过荧光显微镜、zeta电位测量、原子力显微镜和扫描电子显微镜对裸露的和不同修饰的EISCAP表面进行了物理表征。透射电子显微镜用于研究PAH对第二个系统中TMV吸附的影响。最后，通过将青霉素酶固定在TMV表面，实现了一种高灵敏度的TMV辅助EISCAP抗生素生物传感器。通过电容-电压和恒电容方法，在不同青霉素浓度的溶液中对这种PAH/TMV双层修饰的EISCAP生物传感器进行了电化学表征。该生物传感器在0.1 mM至5 mM的浓度范围内，对青霉素的平均灵敏度为113 mV/dec。
