古巴蚊子中自然感染沃尔巴克氏体的首次报告。

First report of natural Wolbachia infections in mosquitoes from Cuba.

作者信息

Ruiz Armando, Gutiérrez-Bugallo Gladys, Rodríguez-Roche Rosmari, Pérez Lissette, González-Broche Raúl, Piedra Luis A, Martínez Liss C, Menéndez Zulema, Vega-Rúa Anubis, Bisset Juan A

机构信息

Department of Vector Control, Center for Research, Diagnostic, and Reference, Institute of Tropical Medicine Pedro Kourí, PAHO-WHO Collaborating Center for Dengue and its Control, Autopista Novia del Mediodía km 6 ½, La Lisa 11 400, Havana, Havana, Cuba.

出版信息

Acta Trop. 2023 Jun;242:106891. doi: 10.1016/j.actatropica.2023.106891. Epub 2023 Mar 11.

DOI:10.1016/j.actatropica.2023.106891

PMID:36907292

Abstract

Mosquitoes are extensively responsible for the transmission of pathogens. Novel strategies using Wolbachia could transform that scenario, since these bacteria manipulate mosquito reproduction, and can confer a pathogen transmission-blocking phenotype in culicids. Here, we screened the Wolbachia surface protein region by PCR in eight Cuban mosquito species. We confirmed the natural infections by sequencing and assessed the phylogenetic relationships among the Wolbachia strains detected. We identified four Wolbachia hosts: Aedes albopictus, Culex quinquefasciatus, Mansonia titillans, and Aedes mediovittatus (first report worldwide). Knowledge of Wolbachia strains and their natural hosts is essential for future operationalization of this vector control strategy in Cuba.

摘要

蚊子在病原体传播中起着广泛的作用。使用沃尔巴克氏体的新策略可能会改变这种情况，因为这些细菌会影响蚊子的繁殖，并能在蚊科昆虫中赋予一种阻止病原体传播的表型。在这里，我们通过聚合酶链反应（PCR）对古巴的八种蚊子进行了沃尔巴克氏体表面蛋白区域的筛选。我们通过测序确认了自然感染情况，并评估了检测到的沃尔巴克氏体菌株之间的系统发育关系。我们确定了四种沃尔巴克氏体宿主：白纹伊蚊、致倦库蚊、骚扰曼蚊和中带伊蚊（全球首次报道）。了解沃尔巴克氏体菌株及其天然宿主对于古巴未来实施这种病媒控制策略至关重要。