Ye Hui-Yu, Shang Zhen-Zi, Zhang Feng-Yun, Zha Xue-Qiang, Li Qiang-Ming, Luo Jian-Ping
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China.
School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, People's Republic of China.
Int J Biol Macromol. 2023 May 1;236:124001. doi: 10.1016/j.ijbiomac.2023.124001. Epub 2023 Mar 11.
This study aimed to explore whether Dendrobium huoshanense stem polysaccharide (cDHPS) ameliorates alcohol-induced gastric ulcer (GU) through the strengthening effect of the gastric mucosal barrier in rats and its potential mechanism. In normal rats, the pretreatment of cDHPS effectively strengthened gastric mucosal barrier by increasing mucus secretion and tight junction protein expression. In GU rats, cDHPS supplementation effectively alleviated alcohol-induced gastric mucosal injury and nuclear factor κB (NF-κB)-driven inflammation by strengthening gastric mucosal barrier. Moreover, cDHPS significantly activated nuclear factor E2-related factor 2 (Nrf2) signaling and promoted antioxidant enzymes activities in both normal and GU rats. These results suggested that the pretreatment of cDHPS could strengthen gastric mucosal barrier to inhibit oxidative stress and NF-κB-driven inflammation induced gastric mucosal injury, which was likely related to the activation of Nrf2 signaling.
本研究旨在探讨霍山石斛茎多糖（cDHPS）是否通过增强大鼠胃黏膜屏障及其潜在机制来改善酒精性胃溃疡（GU）。在正常大鼠中，cDHPS预处理通过增加黏液分泌和紧密连接蛋白表达有效地增强了胃黏膜屏障。在GU大鼠中，补充cDHPS通过增强胃黏膜屏障有效减轻了酒精诱导的胃黏膜损伤和核因子κB（NF-κB）驱动的炎症。此外，cDHPS在正常大鼠和GU大鼠中均显著激活核因子E2相关因子2（Nrf2）信号并促进抗氧化酶活性。这些结果表明，cDHPS预处理可增强胃黏膜屏障，抑制氧化应激和NF-κB驱动的炎症诱导的胃黏膜损伤，这可能与Nrf2信号的激活有关。
