Wang Haonan, Wang Ying, Liu Yuanxi, Xie Jinxin, Zhang Yazhong, Jin Hongyu, Wei Feng, Ma Shuangcheng
State Key Laboratory of Drug Regulatory Science, National Institutes for Food and Drug Control, Beijing 102629, China.
National Institutes for Food and Drug Control, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Foods. 2024 Sep 23;13(18):3011. doi: 10.3390/foods13183011.
This study aimed to analyze the structure of polysaccharides from eight different species and their protective effects on gastric mucosa. Ultraviolet (UV) analysis showed that the contents of eight polysaccharides ranged from 51.89 ± 6.91% to 80.57 ± 11.63%; the degree of acetylation ranged from 0.17 ± 0.03 to 0.48 ± 0.03. High-performance liquid chromatography (HPLC) results showed that these polysaccharides were mainly composed of mannose (Man) and glucose (Glc) with a small amount of galactose (Gal) and arabinose (Ara), and the monosaccharide ratios of different species were different. High-performance size exclusion chromatography-multi angle light scattering-refractive index detector (HPSEC-MALS-RID) showed that the molecular weight (Mw) of all polysaccharides was >1 × 10 Da; had the lowest molecular weight. Subsequently, an ethanol injured GES-1 cell model was constructed to evaluate the gastric mucosal protective potential of polysaccharides from eight different species. The results showed that the protective effect of the low concentration 50 μg/mL DHP treatment group was similar to that of the control group ( > 0.05), and the cell viability could reach 97.32% of that of the control group. Based on the polysaccharide composition, different kinds of have different degrees of migration and repair effects on GES-1 damaged cells, and the effect of DHP is slightly better than that of other varieties (83.13 ± 1.05%). Additionally, polysaccharides alleviated ethanol-induced oxidative stress and inflammatory response in gastric mucosal cells by enhancing the activity of antioxidant enzymes (superoxide dismutase, glutathione peroxidase, catalase) and reducing the levels of malondialdehyde and reactive oxygen species. Overall, DHP can most effectively protect gastric mucosa. These findings enhance our understanding of the relationship between the structure and biological activity of polysaccharides, providing a foundation for the quality control of . Furthermore, these findings offer theoretical support for the development of polysaccharides as nutraceuticals to treat digestive system diseases.
本研究旨在分析八种不同物种多糖的结构及其对胃黏膜的保护作用。紫外（UV）分析表明，八种多糖的含量在51.89±6.91%至80.57±11.63%之间；乙酰化程度在0.17±0.03至0.48±0.03之间。高效液相色谱（HPLC）结果表明，这些多糖主要由甘露糖（Man）和葡萄糖（Glc）组成，含有少量半乳糖（Gal）和阿拉伯糖（Ara），不同物种的单糖比例不同。高效体积排阻色谱-多角度光散射-示差折光检测器（HPSEC-MALS-RID）表明，所有多糖的分子量（Mw）>1×10 Da；分子量最低。随后，构建乙醇损伤的GES-1细胞模型，以评估八种不同物种多糖的胃黏膜保护潜力。结果表明，低浓度50μg/mL DHP处理组的保护作用与对照组相似（>0.05），细胞活力可达对照组的97.32%。基于多糖组成，不同种类的对GES-1损伤细胞有不同程度的迁移和修复作用，DHP的效果略优于其他品种（83.13±1.05%）。此外，多糖通过增强抗氧化酶（超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶）的活性，降低丙二醛和活性氧水平，减轻乙醇诱导的胃黏膜细胞氧化应激和炎症反应。总体而言，DHP能最有效地保护胃黏膜。这些发现加深了我们对多糖结构与生物活性之间关系的理解，为其质量控制提供了基础。此外，这些发现为开发多糖作为治疗消化系统疾病的营养保健品提供了理论支持。
