Institute of International Rivers and Eco-security, Yunnan Key Laboratory of International Rivers and Trans-Boundary Eco-security, Yunnan University, Kunming 650091, People's Republic of China; Yunnan International Joint Research Center for Hydro-Ecology Science & Engineering, Yunnan University, Kunming 650091, People's Republic of China.
Institute of International Rivers and Eco-security, Yunnan Key Laboratory of International Rivers and Trans-Boundary Eco-security, Yunnan University, Kunming 650091, People's Republic of China; Yunnan International Joint Research Center for Hydro-Ecology Science & Engineering, Yunnan University, Kunming 650091, People's Republic of China.
Aquat Toxicol. 2023 May;258:106471. doi: 10.1016/j.aquatox.2023.106471. Epub 2023 Mar 11.
Although the toxicity of silver nanoparticles (AgNPs) has been widely reported, the persistence and reversibility of AgNPs toxicity are poorly understood. In the present work, AgNPs with particle sizes of 5 nm, 20 nm, and 70 nm (AgNPs5, AgNPs20, and AgNPs70) were selected to investigate the nanotoxicity and recovery effects of Chlorella vulgaris in the exposure (72 h) and recovery (72 h) stages using non-targeted metabolomics techniques. The exposure of AgNPs exerted size-dependent effects on several aspects of C. vulgaris physiology, including growth inhibition, chlorophyll content, intracellular silver accumulation, and differential expression of metabolites, and most of these adverse effects were reversible. Metabolomics revealed that AgNPs with small sizes (AgNPs5 and AgNPs20) mainly inhibited glycerophospholipid and purine metabolism, and the effects were reversible. In contrast, AgNPs with large sizes (AgNPs70) reduced amino acid metabolism and protein synthesis by inhibiting aminoacyl-tRNA biosynthesis, and the effects were irreversible, demonstrating the persistence of nanotoxicity of AgNPs. The size-dependent persistence and reversibility of AgNPs toxicity provides new insights to further understand the mechanisms of toxicity of nanomaterials.
尽管银纳米粒子（AgNPs）的毒性已被广泛报道，但人们对 AgNPs 毒性的持久性和可逆性知之甚少。在本工作中，选择粒径为 5nm、20nm 和 70nm 的 AgNPs（AgNPs5、AgNPs20 和 AgNPs70），采用非靶向代谢组学技术，研究了在暴露（72h）和恢复（72h）阶段小球藻对纳米毒性的响应及其恢复效果。AgNPs 的暴露对小球藻的生理机能产生了大小依赖性的影响，包括生长抑制、叶绿素含量、细胞内银积累和代谢物的差异表达，其中大部分的负面影响都是可逆的。代谢组学分析表明，小粒径的 AgNPs（AgNPs5 和 AgNPs20）主要抑制甘油磷脂和嘌呤代谢，其影响是可逆的。相比之下，大粒径的 AgNPs（AgNPs70）通过抑制氨酰-tRNA 生物合成来降低氨基酸代谢和蛋白质合成，其影响是不可逆的，这表明 AgNPs 的纳米毒性具有持久性。AgNPs 毒性的这种大小依赖性的持久性和可逆性为进一步理解纳米材料毒性的机制提供了新的见解。
