Department of Chemistry, University of Quebec in Montreal, CP 8888, Succ. Centre-Ville, Montreal, Quebec, Canada H3C 3P8.
Ecotoxicol Environ Saf. 2012 Apr;78:80-5. doi: 10.1016/j.ecoenv.2011.11.012. Epub 2011 Dec 3.
Freshwater microalga Chlorella vulgaris and marine microalga Dunaliella tertiolecta were used to investigate toxic effects induced by 50 nm silver nanoparticles (AgNPs). To induce AgNPs effect, we exposed Chlorella vulgaris and Dunaliella tertiolecta for 24h to 0-10 mg/L. We showed that growth media had different effects in AgNPs agglomerates' formation. Cellular viability, reactive oxygen species (ROS) formation and lipids peroxidation were employed to assess the toxic effects of AgNPs. AgNPs were able to interact directly with the Chlorella vulgaris cells surface and large aggregates were observed. AgNPs have a negative effect on Chlorella vulgaris and Dunaliella tertiolecta, as manifested by a strong decrease in chlorophyll content, viable algal cells, increased ROS formation and lipids peroxidation. The variability in sensitivity of both algae towards AgNPs was observed. We conclude that AgNPs have a negative effect on aquatic algae and these alterations might have serious consequences on structure and function of aquatic plant communities.
我们分别用淡水微藻小球藻（Chlorella vulgaris）和海洋微藻盐藻（Dunaliella tertiolecta）来研究 50nm 银纳米颗粒（AgNPs）诱导的毒性效应。为了诱导 AgNPs 的效应，我们将小球藻和盐藻分别暴露于 0-10mg/L 的 AgNPs 中 24 小时。结果表明，生长介质对 AgNPs 团聚体的形成有不同的影响。我们采用细胞活力、活性氧（ROS）形成和脂质过氧化来评估 AgNPs 的毒性效应。AgNPs 能够直接与小球藻细胞表面相互作用，并且观察到了大的聚集物。AgNPs 对小球藻和盐藻有负面影响，表现为叶绿素含量、活藻细胞数量的急剧下降，ROS 形成和脂质过氧化增加。两种藻类对 AgNPs 的敏感性存在差异。我们的结论是，AgNPs 对水生藻类有负面影响，这些变化可能对水生植物群落的结构和功能产生严重后果。
