College of Food Science and Technology, Hebei Agricultural University, Baoding, 071000, China.
Mol Nutr Food Res. 2023 May;67(10):e2200577. doi: 10.1002/mnfr.202200577. Epub 2023 Mar 28.
This research work is designed and conducted to explore the joint effect of capsaicin and quercetin and the potential mechanism for the regulation of hyperglycemia.
Insulin-resistance HepG2 cell model and high-fat diet combined with streptozotocin-induced type 2 diabetes mouse model are applied for the investigation. The results illustrate that capsaicin and quercetin can exert hypoglycemic effects via reducing the concentrations of serum lipids of total cholesterol, triglyceride and low-density lipoprotein cholesterol, decreasing hepatic enzyme activities of phosphoenolpyruvate, carboxykinase, and glucose-6-phosphatase, as well as improving the histopathological morphology of liver and pancreas tissues. After administration with capsaicin and quercetin, the relative protein expression abundance of Ras, Raf-1, MEK1/2, and ERK1/2 is obviously attenuated compared to diabetic mice. Capsaicin and quercetin at a ratio of 3:1 are generally determined as the optimal combination.
Capsaicin and quercetin present in the chili pepper fruit can act in a synergistic way to alleviate hyperglycemia. This study provides supporting data for the discovery of novel anti-diabetic components and for the valorization of chili pepper industry.
本研究旨在探索辣椒素和槲皮素的联合作用及其调节高血糖的潜在机制。
采用胰岛素抵抗 HepG2 细胞模型和高脂饮食联合链脲佐菌素诱导的 2 型糖尿病小鼠模型进行研究。结果表明，辣椒素和槲皮素通过降低血清总胆固醇、甘油三酯和低密度脂蛋白胆固醇浓度，降低磷酸烯醇丙酮酸、羧基激酶和葡萄糖-6-磷酸酶的肝酶活性，以及改善肝和胰腺组织的组织病理学形态，发挥降血糖作用。与糖尿病小鼠相比，给予辣椒素和槲皮素后，Ras、Raf-1、MEK1/2 和 ERK1/2 的相对蛋白表达丰度明显降低。辣椒素和槲皮素的比例为 3:1 时通常被确定为最佳组合。
辣椒果实中的辣椒素和槲皮素具有协同作用，可缓解高血糖。本研究为发现新型抗糖尿病成分和辣椒产业增值提供了数据支持。
