Rishabh Dwivedi, Athira Ajith, Preetha Radhakrishnan, Nagamaniammai G
Department of Food Process Engineering, School of Bioengineering, The College of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, Chengalpattu District, Chennai, Tamil Nadu 603203 India.
J Food Sci Technol. 2023 Mar;60(3):916-924. doi: 10.1007/s13197-021-05259-2. Epub 2021 Sep 6.
Probiotic strains isolated from - a traditional Indian fermented food-and fermented carrot juice, were evaluated for their probiotic properties and analyzed probiotic properties such as lactic acid production, NaCl tolerance, and acid tolerance for all the isolated strains. Most isolated strains were susceptible to antibiotics, and cell-free extracts showed potential antioxidant activity and observed antagonistic properties against foodborne pathogens such as ATCC 6539 ATCC 25922 ATCC 12022, and ATCC 29213. The isolate (K13), was incorporated in carrot juice and microencapsulated by freeze-drying and spray drying. Gum Arabic and maltodextrin were used as coating materials. The physicochemical properties, such as bulk density, moisture content, water activity and color of the powders, and the survivability of probiotic bacteria, were studied on storage. The freeze-dried probiotic formulation is suggested over spray-dried formulation for further use, since it had good storage stability up to one month (6-7 Log CFU/g).
从一种传统印度发酵食品和发酵胡萝卜汁中分离出的益生菌菌株,对其益生菌特性进行了评估,并分析了所有分离菌株的乳酸产生、耐氯化钠和耐酸等益生菌特性。大多数分离菌株对抗生素敏感,无细胞提取物显示出潜在的抗氧化活性,并对食源性病原体如ATCC 6539、ATCC 25922、ATCC 12022和ATCC 29213具有拮抗特性。分离株(K13)被添加到胡萝卜汁中,并通过冷冻干燥和喷雾干燥进行微胶囊化。阿拉伯胶和麦芽糊精用作包衣材料。研究了粉末的堆积密度、水分含量、水分活度和颜色等物理化学性质以及益生菌在储存时的存活率。建议进一步使用冷冻干燥的益生菌制剂而非喷雾干燥制剂,因为它在长达一个月的时间内具有良好的储存稳定性(6 - 7 Log CFU/g)。