Bhat Ishani, Jose Nimmy Mol, Mamatha Bangera Sheshappa
Nitte University Center for Science Education and Research (NUCSER), Nitte (Deemed to Be University), Paneer Campus, Deralakatte, Mangaluru, Karnataka 575018 India.
J Food Sci Technol. 2023 Mar;60(3):987-995. doi: 10.1007/s13197-022-05430-3. Epub 2022 Apr 15.
Pre-processing treatments performed on lutein sources can cause it to degrade, generating superfluous metabolites and lowering lutein's bioactivity. However, evidences suggesting extent of reduction in functional stability of lutein on exposure to such treatment conditions are nil. This study is first of its kind, where we attempted to gain clarity on the extent of degradation caused by the changes in temperature (40-100 °C), pH (2-8) and duration of such treatments. Increase (3.9 folds) in lutein loss within an hour at 40 °C occurred when pH was lowered from 8 to 2. Increase (1.7 folds) in lutein loss at neutral pH and 40 °C occurred when duration of exposure was increased from 1 to 4 h. Besides, lutein loss significantly increased on rising the temperature by every 10 °C. The functional stability of lutein in relation to its degradation was also studied by monitoring its radical scavenging activity. While lutein is highly unstable, lutein structure and its respective bioactivity can be significantly ( < 0.05) retained (< 12.44% and > 54.87% respectively) by maintaining the operating conditions at higher pH (7-8) and lower temperatures (40-50 °C) for a short period of time (< 1 h).
The online version contains supplementary material available at 10.1007/s13197-022-05430-3.
对叶黄素来源进行的预处理可能会导致其降解，产生多余的代谢产物并降低叶黄素的生物活性。然而，关于叶黄素在暴露于此类处理条件下功能稳定性降低程度的证据却不存在。本研究尚属首次，我们试图弄清楚温度（40 - 100°C）、pH值（2 - 8）变化以及此类处理持续时间所导致的降解程度。当pH值从8降至2时，40°C下一小时内叶黄素损失增加（3.9倍）。当中性pH值和40°C时，暴露时间从1小时增加到4小时，叶黄素损失增加（1.7倍）。此外，温度每升高10°C，叶黄素损失显著增加。还通过监测其自由基清除活性研究了叶黄素降解与其功能稳定性的关系。虽然叶黄素非常不稳定，但通过在较高pH值（7 - 8）和较低温度（40 - 50°C）下短时间（<1小时）维持操作条件，叶黄素结构及其相应的生物活性可得到显著保留（分别<12.44%和>54.87%，P<0.05）。
在线版本包含可在10.1007/s13197 - 022 - 05430 - 3获取的补充材料。
