Tuo Ying, Wang Mingrui, Yu Yiwei, Li Yixiao, Hu Xingyuan, Wu Long, Zhang Zongpei, Zhou Hui, Li Xiang
College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China.
Dalian Jinshiwan Laboratory, Dalian 116034, China.
Mar Drugs. 2025 Jul 30;23(8):311. doi: 10.3390/md23080311.
Poor solubility and bioavailability have limited the application of fucoxanthin in drug and functional food processing. In order to encapsulate fucoxanthin in delivery systems, in this study, cellulose was isolated from industrial brown algae residues and high-pressure homogenized into cellulose nanofibrils (CNFs). Then, fucoxanthin was encapsulated into the Pickering emulsion stabilized by the CNFs. The effect of high-pressure homogenization on the characteristics of cellulose and the stability of fucoxanthin emulsion was evaluated. The results indicated that CNFs prepared at 105 MPa had a diameter of 87 nm and exhibited high zeta potential and thermal stability. Encapsulation efficiency peaked at 70.8% with 1.0 mg/mL fucoxanthin, and after three freeze-thaw cycles the encapsulation efficiency was higher than 60%. The DPPH scavenging activity after 12 days' storage at 4 °C was still 42%. Furthermore, the Pickering emulsion with 1.0 mg/mL fucoxanthin showed high stability and antioxidant activity under different pH values, salinity, temperature, and UV light exposure duration. The CNFs effectively protected fucoxanthin from degradation, offering a novel delivery system for marine bioactive compounds. To the best of our knowledge, this is the first study on the fucoxanthin delivery system of Pickering emulsion stabilized by the CNFs. Such emulsion might benefit the encapsulation and release of bioactive components in marine drugs.
溶解性和生物利用度差限制了岩藻黄质在药物和功能性食品加工中的应用。为了将岩藻黄质封装到递送系统中，在本研究中，从工业褐藻残渣中分离出纤维素，并通过高压均质法将其制成纤维素纳米纤维（CNFs）。然后，将岩藻黄质封装到由CNFs稳定的Pickering乳液中。评估了高压均质对纤维素特性和岩藻黄质乳液稳定性的影响。结果表明，在105 MPa下制备的CNFs直径为87 nm，具有较高的ζ电位和热稳定性。当岩藻黄质浓度为1.0 mg/mL时，包封率峰值为70.8%，经过三次冻融循环后，包封率高于60%。在4℃储存12天后，DPPH清除活性仍为42%。此外，含有1.0 mg/mL岩藻黄质的Pickering乳液在不同的pH值、盐度、温度和紫外线照射时间下均表现出高稳定性和抗氧化活性。CNFs有效地保护了岩藻黄质不被降解，为海洋生物活性化合物提供了一种新型递送系统。据我们所知，这是第一项关于由CNFs稳定的Pickering乳液岩藻黄质递送系统的研究。这种乳液可能有利于海洋药物中生物活性成分的封装和释放。
