血清中抗甲状腺球蛋白和甲状腺微粒体自身抗体同时测定的酶免疫分析

Enzyme immunoassay for simultaneous measurement of autoantibodies against thyroglobulin and thyroid microsome in serum.

作者信息

Ng M L, Rajna A, Khalid B A

机构信息

Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur.

出版信息

Clin Chem. 1987 Dec;33(12):2286-8.

PMID:3690847

Abstract

A combined enzyme immunoassay (micro-ELISA) technique was established for measuring autoantibodies against thyroglobulin and thyroid microsome, involving the immuno-dot blot technique. Thyroglobulin and thyroid microsome antigens (1 g/L each) prepared from normal thyroids were spotted separately onto nitrocellulose membrane filter discs. Results by this method and those by immunofluorescence correlated well. The percentages of confirmed positives were 30% and 48% and the negatives were 58% and 46% (n = 50) for thyroglobulin and microsome, respectively. Testing these samples by gelatin agglutination gave a high percentage of false positives (up to 20%, n = 128) and hemagglutination testing yielded a high percentage of false negatives (up to 20%, n = 45). The titer of autoantibodies by the micro-ELISA technique was greater than by agglutination. This technique is highly specific and sensitive.

摘要

建立了一种结合酶免疫测定法（微量酶联免疫吸附测定）来检测抗甲状腺球蛋白和甲状腺微粒体自身抗体，该方法涉及免疫斑点印迹技术。将从正常甲状腺制备的甲状腺球蛋白和甲状腺微粒体抗原（各1 g/L）分别点样于硝酸纤维素膜滤片上。该方法与免疫荧光法的结果相关性良好。甲状腺球蛋白和微粒体的确诊阳性率分别为30%和48%，阴性率分别为58%和46%（n = 50）。用明胶凝集试验检测这些样本出现了高比例的假阳性（高达20%，n = 128），而血凝试验则出现了高比例的假阴性（高达20%，n = 45）。通过微量酶联免疫吸附测定技术检测的自身抗体滴度高于凝集试验。该技术具有高度特异性和敏感性。