Goodburn R, Williams D L, Marks V
Clin Chim Acta. 1982 Mar 12;119(3):291-7. doi: 10.1016/0009-8981(82)90342-4.
Thyroid microsomes for use in assay systems for anti-thyroid-microsomal antibodies are normally prepared by ultracentrifugation. By means of gel filtration studies we show that preparations made in this way are invariably contaminated with thyroglobulin. A further purification by gel filtration is described which enables the preparation of thyroid microsomes of sufficient purity for use in a micro-ELISA method for the measurement of anti-thyroid-microsomal antibodies. Microsomes prepared in this way would also be suitable for haemagglutination and radioassays, which are also affected by thyroglobulin contamination.
用于抗甲状腺微粒体抗体检测系统的甲状腺微粒体通常通过超速离心制备。通过凝胶过滤研究，我们发现以这种方式制备的制剂总是被甲状腺球蛋白污染。本文描述了一种通过凝胶过滤进一步纯化的方法，该方法能够制备出纯度足够高的甲状腺微粒体，用于微量酶联免疫吸附测定法检测抗甲状腺微粒体抗体。以这种方式制备的微粒体也适用于血细胞凝集和放射分析，这些方法也会受到甲状腺球蛋白污染的影响。
