Shenyang Agricultural University, Shenyang, Liaoning, China.
Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, Shenyang, Liaoning, China.
PeerJ. 2023 Mar 7;11:e14938. doi: 10.7717/peerj.14938. eCollection 2023.
In this study, a pBI121- plant expression vector was constructed on the basis of obtaining the full-length sequence of the gene from , which was then used for genetic transformation inflorescence infection using wild-type and mutants as transgenic receptors. Seeds of positive plants with high expression of were collected and sowed till the homozygous T3 regeneration plants were obtained. Then the expression of flowering-related genes (, , and ) in T3 generation plants were analyzed and the results showed that gene overexpression promoted the expression of flowering-related genes and resulted in earlier flowering in . The plants of -transformed and -transformed mutants appeared shorter leaves, longer fruit pods, and fewer cauline leaves than those of wild-type and the mutants plants, respectively. In addition, some secondary branches in the transgenic plants converted into inflorescences, which indicated that the overexpression of promoted the transition from vegetative growth to reproductive growth, and compensate the phenotypic defects of mutant partially. The results provides a scientific reference for formulating reasonable genetic improvement strategies such as shortening childhood, improving yield and quality, and breeding desirable varieties, which have important guiding significance in production.
在这项研究中，我们从 中获得了 基因的全长序列，并在此基础上构建了 pBI121-植物表达载体，然后利用野生型 和 突变体作为转基因受体，通过花序浸染法进行遗传转化。收集并播种高表达 的 阳性植株的种子，直至获得纯合的 T3 再生植株。然后分析 T3 代植株中与开花相关的基因（、、和 ）的表达情况，结果表明，基因的过表达促进了开花相关基因的表达，导致 提前开花。与野生型和 突变体植株相比，转化了 -和 -的 突变体植株的叶片变短，果荚变长，茎生叶变少。此外，一些转基因植株的次级分枝转化为花序，这表明 基因的过表达促进了营养生长向生殖生长的转变，部分补偿了 突变体的表型缺陷。该研究结果为制定合理的遗传改良策略提供了科学参考，如缩短生育期、提高产量和品质、培育理想品种等，在生产中具有重要的指导意义。
