Zhou Wei, Wieczorek Martyna N, Jiang Runshan Will, Pawliszyn Janusz
Department of Chemistry, University of Waterloo, Waterloo, ON, N2L 3G1, Canada.
Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Poznań, Poland.
J Pharm Anal. 2023 Feb;13(2):216-222. doi: 10.1016/j.jpha.2022.10.004. Epub 2022 Nov 9.
The direct coupling of solid-phase microextraction (SPME) to mass spectrometry (MS) (SPME-MS) has proven to be an effective method for the fast screening and quantitative analysis of compounds in complex matrices such as blood and plasma. In recent years, our lab has developed three novel SPME-MS techniques: SPME-microfluidic open interface-MS (SPME-MOI-MS), coated blade spray-MS (CBS-MS), and SPME-probe electrospray ionization-MS (SPME-PESI-MS). The fast and high-throughput nature of these SPME-MS technologies makes them attractive options for point-of-care analysis and anti-doping testing. However, all these three techniques utilize different SPME geometries and were tested with different MS instruments. Lack of comparative data makes it difficult to determine which of these methodologies is the best option for any given application. This work fills this gap by making a comprehensive comparison of these three technologies with different SPME devices including SPME fibers, CBS blades, and SPME-PESI probes and SPME-liquid chromatography-MS (SPME-LC-MS) for the analysis of drugs of abuse using the same MS instrument. Furthermore, for the first time, we developed different desorption chambers for MOI-MS for coupling with SPME fibers, CBS blades, and SPME-PESI probes, thus illustrating the universality of this approach. In total, eight analytical methods were developed, with the experimental data showing that all the SPME-based methods provided good analytical performance with of linearities larger than 0.9925, accuracies between 81% and 118%, and good precision with an RSD% 13%.
事实证明，将固相微萃取（SPME）直接与质谱（MS）联用（SPME-MS）是一种用于快速筛查和定量分析血液和血浆等复杂基质中化合物的有效方法。近年来，我们实验室开发了三种新型的SPME-MS技术：SPME-微流控开放界面-MS（SPME-MOI-MS）、涂层刀片喷雾-MS（CBS-MS）和SPME-探针电喷雾电离-MS（SPME-PESI-MS）。这些SPME-MS技术的快速和高通量特性使其成为即时检测分析和反兴奋剂检测的有吸引力的选择。然而，这三种技术都采用了不同的SPME几何结构，并使用不同的质谱仪器进行了测试。缺乏比较数据使得难以确定这些方法中哪一种是任何给定应用的最佳选择。这项工作通过使用同一质谱仪对这三种技术与不同的SPME装置（包括SPME纤维、CBS刀片和SPME-PESI探针）以及SPME-液相色谱-MS（SPME-LC-MS）进行全面比较，填补了这一空白。此外，我们首次为MOI-MS开发了不同的解吸室，用于与SPME纤维、CBS刀片和SPME-PESI探针联用，从而说明了这种方法具有通用性。总共开发了八种分析方法，实验数据表明，所有基于SPME的方法都具有良好的分析性能，线性度大于0.9925，准确度在81%至118%之间，精密度良好，相对标准偏差（RSD%）小于13%。
