Xu Xifei, Liu Wenjian, Niu Honghong, Hua Mei, Su Ying, Miao Xinyu, Chi Yanping, Xu Hongyan, Wang Jinghui, Sun Mubai, Li Da
Department of Food Science and Engineering, College of Agriculture, Yanbian University, Yanji, China.
Laboratory of Food Microbiology, Institute of Agro-product Process, Jilin Academy of Agricultural Science, Changchun, China.
Front Nutr. 2023 Feb 24;10:1125720. doi: 10.3389/fnut.2023.1125720. eCollection 2023.
Tofu whey wastewater (TWW) is the wastewater of tofu processing, which is rich in a variety of nutrients. can make full use of TWW to ferment and reproduce yeast cells, produce carotenoids and other nutrients, improve the utilization value of TWW, and reduce environmental pollution and resource waste.
In this study, the nutrient composition changes of TWW treated by were analyzed to reformulate TWW medium, and the optimal composition and proportion of TWW medium that can improve the biomass and carotenoids production of were explored. Meanwhile, the liquid obtained under these conditions was used to prepare biological feed for laying hens, and the effect of growing on TWW as substrate on laying performance and egg quality of laying hens were verified.
The results showed that the zinc content of TWW after fermentation increased by 62.30%, the phosphorus content decreased by 42.31%, and the contents of vitamin B1, B2 and B6 increased to varying degrees. The optimal fermentation conditions of in the TWW medium were as follow: the initial pH was 6.40, the amount of soybean oil, glucose and zinc ions was 0.80 ml/L, 16.32 g/L, and 20.52 mg/L, respectively. Under this condition, the biomass of reached 2.23 g/L, the carotenoids production was 832.86 μg/g, and the number of effective viable yeast count was 7.08 × 10 cfu/ml. In addition, the laying performance and egg quality of laying hens fed biological feed were improved.
In this study, we analyzed the composition changes of TWW, optimized the fermentation conditions of in TWW medium, explored the influence of utilizing TWW on laying layers, and provided a new idea for the efficient utilization of TWW.
豆腐乳清废水(TWW)是豆腐加工过程中产生的废水,富含多种营养成分。充分利用TWW发酵繁殖酵母细胞,生产类胡萝卜素等营养物质,可提高TWW的利用价值,减少环境污染和资源浪费。
本研究分析了经处理的TWW营养成分变化,以重新配制TWW培养基,探索能提高其生物量和类胡萝卜素产量的TWW培养基最佳组成和比例。同时,将在这些条件下获得的液体用于制备蛋鸡生物饲料,验证以TWW为底物生长对蛋鸡产蛋性能和蛋品质的影响。
结果表明,发酵后TWW的锌含量增加了62.30%,磷含量降低了42.31%,维生素B1、B2和B6的含量均有不同程度增加。TWW培养基中该菌的最佳发酵条件如下:初始pH为6.40,大豆油、葡萄糖和锌离子的用量分别为0.80 ml/L、16.32 g/L和20.52 mg/L。在此条件下,该菌的生物量达到2.23 g/L,类胡萝卜素产量为832.86 μg/g,有效活酵母数为7.08×10 cfu/ml。此外,饲喂该生物饲料的蛋鸡产蛋性能和蛋品质得到改善。
本研究分析了TWW的成分变化,优化了TWW培养基中该菌的发酵条件,探索了该菌利用TWW对蛋鸡的影响,为TWW的高效利用提供了新思路。