Utama Gemilang Lara, Suraloka Mahardhika Puspa Arum, Rialita Tita, Balia Roostita Lobo
Master Program of Agro-Industrial Technology, Faculty of Agro-Industrial Technology, University of Padjadjaran, Jatinangor 45363, Indonesia.
Centre for Environment and Sustainability Science, University of Padjadjaran, Bandung 40134, Indonesia.
Foods. 2021 Oct 28;10(11):2619. doi: 10.3390/foods10112619.
Yeast can be isolated from tofu wastewater and the cell wall in the form of β-glucan can act as a natural decontaminant agent. This study aimed to isolate and characterize native yeast from tofu wastewater, which can be extracted to obtain β-glucan and then identify the yeast and its β-glucan activity regarding antifungal ability against and aflatoxin-reducing activity towards aflatoxin B1 (AFB1) and B2 (AFB2). Tofu wastewater native yeast was molecularly identified, and the growth observed based on optical density for 96 h and the pH also measured. β-glucan was extracted from native yeast cell walls with the acid-base method and then the inhibition activity towards was tested using the well diffusion method and microscopic observation. AFB1 and AFB2 reduction were identified using HPLC LC-MS/MS. The results showed that the native yeast isolated was with a β-glucan yield of 6.59%. and its β-glucan showed an inhibition zone against of 11.33 ± 4.93 and 7.33 ± 3.51 mm, respectively. Total aflatoxin-reducing activity was also shown by of 26.85 ± 2.87%, and β-glucan of 27.30 ± 1.49%, while AFB1- and AFB2-reducing activity by was 36.97 ± 3.07% and 27.13 ± 1.69%, and β-glucan was 27.13 ± 1.69% and 32.59 ± 4.20%, respectively.
酵母可从豆腐废水中分离得到，其细胞壁中的β-葡聚糖可作为一种天然去污剂。本研究旨在从豆腐废水中分离并鉴定本地酵母，提取其β-葡聚糖，然后鉴定该酵母及其β-葡聚糖对[未提及的某种真菌]的抗真菌能力以及对黄曲霉毒素B1（AFB1）和B2（AFB2）的黄曲霉毒素降低活性。对豆腐废水本地酵母进行了分子鉴定，基于光密度观察其96小时的生长情况，并测量了pH值。采用酸碱法从本地酵母细胞壁中提取β-葡聚糖，然后用打孔扩散法和显微镜观察测试其对[未提及的某种真菌]的抑制活性。使用HPLC LC-MS/MS鉴定AFB1和AFB2的降低情况。结果表明，分离得到的本地酵母为[未提及的某种酵母]，β-葡聚糖产率为6.59%。其β-葡聚糖对[未提及的某种真菌]的抑菌圈分别为11.33±4.93和7.33±3.51毫米。[未提及的某种酵母]的总黄曲霉毒素降低活性为26.85±2.87%，β-葡聚糖为27.30±1.49%，而[未提及的某种酵母]对AFB1和AFB2的降低活性分别为36.97±3.07%和27.13±1.69%，β-葡聚糖分别为27.13±1.69%和32.59±4.20%。
