Wu Yuxing, Wang Yajiao, Han Sen, Li Qiusheng, Kong Lingxiao
Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Integrated Pest Management Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Baoding, China.
Front Plant Sci. 2023 Feb 22;14:1132507. doi: 10.3389/fpls.2023.1132507. eCollection 2023.
is a soil-borne pathogen that is capable of causing a highly destructive crown disease in wheat. Secondary metabolites (SMs), especially deoxynivalenol (DON), are the primary virulence factors during infection. Here, we characterised the global regulator , an orthologue of LaeB protein function, to regulate the SM in . Through the utility of the gene targeting approach, we found that the vegetative growth of the deletion mutant was drastically reduced compared to that of the wild type. was also important for conidiation because the deletion mutant formed fewer conidia in induced medium. In addition, the sensitivity of the deletion mutant to the cell wall integrity inhibitor was decreased, while its growth was more severely inhibited by the cell membrane inhibitor sodium dodecyl sulfate (SDS) than that of the wild type. More importantly, the virulence was decreased when the deletion mutant was inoculated onto the wheat stem base or head. Through genome-wide gene expression profiling, was found to regulate several processes related to the above phenotypes such as the carbohydrate metabolic process, which is an integral and intrinsic component of membranes, especially SMs. Furthermore, the generation of DON was impaired in the deletion mutant ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) assay. These results showed that plays an important role in the growth, development, and maintenance of the cell wall, and in membrane integrity. More importantly, is required for SMs and full virulence in .
是一种土传病原体，能够在小麦中引发极具破坏性的冠腐病。次生代谢产物（SMs），尤其是脱氧雪腐镰刀菌烯醇（DON），是感染过程中的主要毒力因子。在此，我们对全局调控因子进行了表征，它是LaeB蛋白功能的直系同源物，用于调控中的SMs。通过基因靶向方法的应用，我们发现与野生型相比，缺失突变体的营养生长大幅降低。对于分生孢子形成也很重要，因为缺失突变体在诱导培养基中形成的分生孢子较少。此外，缺失突变体对细胞壁完整性抑制剂的敏感性降低，而其生长受到细胞膜抑制剂十二烷基硫酸钠（SDS）的抑制比野生型更严重。更重要的是，当将缺失突变体接种到小麦茎基部或穗部时，其毒力降低。通过全基因组基因表达谱分析，发现可调控与上述表型相关的多个过程，如碳水化合物代谢过程，这是膜尤其是SMs不可或缺的内在组成部分。此外，在缺失突变体的超高效液相色谱串联质谱（UPLC-MS/MS）分析中，DON的产生受到损害。这些结果表明，在细胞壁的生长、发育和维持以及膜完整性方面发挥着重要作用。更重要的是，对于中的SMs和完全毒力是必需的。
