乌干达3至89岁HIV阴性个体外周血单个核细胞和口腔液中爱泼斯坦-巴尔病毒与卡波西肉瘤相关疱疹病毒病毒载量的比较
Comparison of Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus viral load in peripheral blood mononuclear cells and oral fluids of HIV-negative individuals aged 3 to 89 years from Uganda.
作者信息
Nalwoga Angela, Marshall Vickie, Miley Wendell, Labo Nazzarena, Whitby Denise, Newton Robert, Rochford Rosemary
机构信息
University of Colorado, Anschutz Medical Campus.
Leidos Biomedical Research, Inc, Frederick National Laboratory for Cancer Research.
出版信息
Res Sq. 2023 Mar 2:rs.3.rs-2613771. doi: 10.21203/rs.3.rs-2613771/v1.
We previously found that age, sex, and malaria were associated with KSHV viral load in individuals from Uganda. In this study, we have evaluated factors associated with presence of EBV DNA in blood and oral fluids among the same individuals, using the same biological samples. Overall, 74% of oral fluids samples and 46% of PBMCs had detectable EBV, compared to 24% and 11% for KSHV respectively Individuals with EBV in PBMCs were more likely to have KSHV in PBMCs (P=0.016). The peak age for detection of EBV in oral fluids was 3-5 years while that of KSHV was 6-12 years. In PBMCs, the peak age for detection of EBV was 66+ years and KSHV was 3-5 years. Individuals with malaria had higher levels of EBV in PBMCs compared to malaria-negative individuals (P=0.002). In summary, our results show that younger age and malaria are associated with higher levels of EBV and KSHV in PBMCs suggesting malaria impacts immunity to EBV and KSHV.
我们之前发现，在乌干达个体中，年龄、性别和疟疾与卡波西肉瘤相关疱疹病毒（KSHV）病毒载量有关。在本研究中，我们使用相同的生物样本，评估了同一人群血液和口腔液体中EB病毒（EBV）DNA存在情况的相关因素。总体而言，74%的口腔液体样本和46%的外周血单核细胞（PBMC）中可检测到EBV，相比之下，KSHV分别为24%和11%。PBMC中存在EBV的个体更有可能在PBMC中存在KSHV（P = 0.016）。口腔液体中EBV检测的高峰年龄为3至5岁，而KSHV为6至12岁。在PBMC中，EBV检测的高峰年龄为66岁以上，KSHV为3至5岁。与疟疾阴性个体相比，患疟疾的个体PBMC中EBV水平更高（P = 0.002）。总之，我们的结果表明，年龄较小和疟疾与PBMC中较高水平的EBV和KSHV有关，提示疟疾会影响对EBV和KSHV的免疫力。
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