Viral Oncology Program, Sidney Kimmel Cancer Center, Baltimore, Maryland, USA.
J Infect Dis. 2011 Dec 1;204(11):1683-91. doi: 10.1093/infdis/jir645. Epub 2011 Oct 11.
Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).
To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.
In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.
The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.
爱泼斯坦-巴尔病毒（EBV）是一种普遍存在的疱疹病毒，卡波济肉瘤相关疱疹病毒（KSHV）的血清流行率有限。这两种病毒都与恶性肿瘤有关，在同时感染人类免疫缺陷病毒 1 型（HIV-1）的个体中，这些恶性肿瘤的发病率更高。
为了全面了解这些病毒的体液免疫反应，我们生成了一个蛋白质芯片，该芯片显示了从酵母中纯化的 174 种 EBV 和 KSHV 多肽。我们检测了来自健康志愿者以及患有 B 细胞淋巴瘤或 AIDS 相关卡波济肉瘤或淋巴瘤的患者血浆中的 EBV 和 KSHV 抗体反应。
除了常见的研究抗原外，还经常检测到针对 KSHV ORF38 和 EBV BBRF 和 BGLF2 和 BNRF1 以及 EBV 早期裂解蛋白 BRRF1 和 BORF2 的 IgG 反应。EBV vIL-10 蛋白特别容易被血浆 IgA 识别。对 EBV 抗原的最强 IgG 反应发生在 HIV-1 阳性患者中。在血浆中病毒 DNA 载量与抗体谱之间未观察到明显相关性。
蛋白质芯片为全面筛选提供了一个敏感的平台；确定了新的、经常被识别的病毒抗原；并显示了与同一患者中的 KSHV 相比，对 EBV 产生了更广泛的体液免疫反应。
