“CORE”：一种通过基质辅助激光解吸电离飞行时间质谱在正离子模式下快速鉴定耐黏菌素菌株的新检测方法。
"CORE" a new assay for rapid identification of COlistin REsistant strains by MALDI-TOF MS in positive-ion mode.
作者信息
Foglietta Gianluca, De Carolis Elena, Mattana Giordana, Onori Manuela, Agosta Marilena, Niccolai Claudia, Di Pilato Vincenzo, Rossolini Gian Maria, Sanguinetti Maurizio, Perno Carlo Federico, Bernaschi Paola
机构信息
Microbiology Unit and Diagnostic Immunology, Bambino Gesù Pediatric Hospital, IRCCS, Rome, Italy.
Microbiology Unit, Department of Laboratory Sciences and Infectious Diseases, Fondazione Policlinico Universitario A. Gemelli, IRCCS, Rome, Italy.
出版信息
Front Microbiol. 2023 Feb 22;14:1045289. doi: 10.3389/fmicb.2023.1045289. eCollection 2023.
Due to the global spread of pan resistant organisms, colistin is actually considered as one of the last resort antibiotics against MDR and XDR bacterial infections. The emergence of colistin resistant strains has been observed worldwide in Gram-negative bacteria, such as and especially in in association with increased morbidity and mortality. This landscape implies the exploration of novel assays able to target colistin resistant strains rapidly. In this study, we developed and evaluated a new MALDI-TOF MS assay in positive-ion mode that allows quantitative or qualitative discrimination between colistin susceptible (18) or resistant (32) strains in 3 h by using the "Autof MS 1000" mass spectrometer. The proposed assay, if integrated in the diagnostic workflow, may be of help for the antimicrobial stewardship and the control of the spread of colistin resistant isolates in hospital settings.
由于泛耐药菌在全球范围内传播，黏菌素实际上被视为对抗多重耐药和广泛耐药细菌感染的最后手段之一。在革兰氏阴性菌中，全球范围内均已观察到黏菌素耐药菌株的出现，尤其是在 ，其与发病率和死亡率的增加相关。这种情况意味着需要探索能够快速靶向黏菌素耐药菌株的新型检测方法。在本研究中，我们开发并评估了一种新的正离子模式基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）检测方法，该方法使用“Autof MS 1000”质谱仪，可在3小时内对黏菌素敏感（18株）或耐药（32株） 菌株进行定量或定性区分。如果将所提出的检测方法整合到诊断工作流程中，可能有助于抗菌药物管理以及控制医院环境中黏菌素耐药菌株的传播。
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