Meclat Vanessa Yvonne B, Ancheta Keith Sigfred B, Adao Davin Edric V, Rivera Windell L
Institute of Biology, College of Science, University of the Philippines Diliman, 1101 Quezon City, Philippines.
J Parasit Dis. 2023 Mar;47(1):192-197. doi: 10.1007/s12639-022-01554-7. Epub 2022 Nov 28.
is an intestinal protist commonly identified in human and animal feces. At present, there are 28 proposed subtypes (STs) identified based on the small subunit rRNA gene, 13 of which are found in both humans and animals. In general, nonmammalian and avian groups are infected by the nonmammalian and avian subtypes (NMASTs). However, NMASTs were also isolated from mammalian hosts, suggesting possible cross-contamination and transmission from nonmammalian and avian hosts to other animals and, potentially, humans. Thus, this study determined the possible relationship between NMAST sequences and conventional STs to provide new insights into classification, identification, and epidemiological significance. Phylogenetic trees were constructed using three statistical models, namely, Maximum Likelihood, Neighbor Joining, and Maximum Parsimony, based on the 30 NMAST sequences. The NMAST sequences formed groups clustered closely with other NMAST subtrees. Most sequences of nonmammalian and avian isolates formed distinct monophyletic clades based on their NMAST classification, with some clustering with mammalian and avian STs. These results indicate the close relationship between isolated from mammalian and avian hosts and nonmammalian and avian hosts.
The online version contains supplementary material available at (10.1007/s12639-022-01554-7).
是一种常见于人类和动物粪便中的肠道原生生物。目前，基于小亚基rRNA基因已鉴定出28种提议的亚型（STs），其中13种在人类和动物中均有发现。一般来说，非哺乳动物和鸟类群体感染非哺乳动物和鸟类亚型（NMASTs）。然而，也从哺乳动物宿主中分离出了NMASTs，这表明可能存在交叉污染以及从非哺乳动物和鸟类宿主传播到其他动物，甚至可能传播给人类。因此，本研究确定了NMAST序列与传统STs之间的可能关系，以提供关于分类、鉴定和流行病学意义的新见解。基于30个NMAST序列，使用三种统计模型，即最大似然法、邻接法和最大简约法构建了系统发育树。NMAST序列形成的组与其他NMAST子树紧密聚类。大多数非哺乳动物和鸟类分离株的序列根据其NMAST分类形成了不同的单系分支，有些与哺乳动物和鸟类的STs聚类。这些结果表明从哺乳动物和鸟类宿主分离出的与非哺乳动物和鸟类宿主之间存在密切关系。
在线版本包含可在(10.1007/s12639-022-01554-7)获取的补充材料。
