Madkour Bahaa S, Karmi Mohamed, Youssef Menna Allah, Abdelraouf Aml, Abdel-Rady Ahmed
Department of Animal Medicine, Facultyof VeterinaryMedicine, Aswan University, Aswan, Egypt.
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt.
J Parasit Dis. 2023 Mar;47(1):124-130. doi: 10.1007/s12639-022-01547-6. Epub 2022 Nov 10.
The present study was carried out to investigate the epidemiological and clinical status of bovine Theileriosis in Aswan governorate. During a 2-year study, 265 cattle were clinically suspected upon careful clinical examination as () infected animals. Conventional diagnosis based on blood and lymph smears examinations showed that, the prevalence of Tropical Theileriosis in cattle in Aswan governorate was 56 (21.13%). Stained blood smears showed the presence of macro and/or micro-schizonts inside lymphocyte (Koch's blue bodies. Intraerythrocytic stages of piroplasms inside RBCs. Polymerase chain reactions of merozoite-piroplasms surface antigen Targeting gene: (Tams-1), revealed positive 29 (58%) animals confirmed by visualization of specific bands at 768 bp. Positive results could be detected in suspected cattle that showed positive or negative blood smear results that confirmed the high sensitivity of the PCR technique compared with the conventional method for diagnosis of bovine tropical Theileriosis. PCR proved to be a highly sensitive and accurate method for diagnosis of bovine tropical theileriosis especially in the detection of samples that was negative on blood and lymph smears.
本研究旨在调查阿斯旺省牛泰勒虫病的流行病学和临床状况。在一项为期2年的研究中，经过仔细的临床检查，有265头牛被临床怀疑为（）感染动物。基于血液和淋巴涂片检查的传统诊断表明，阿斯旺省牛群中热带泰勒虫病的患病率为56（21.13%）。染色血涂片显示淋巴细胞内存在大裂殖体和/或小裂殖体（科赫氏蓝体），红细胞内存在梨形虫的红细胞内阶段。针对裂殖子-梨形虫表面抗原靶向基因（Tams-1）的聚合酶链反应显示，29头（58%）动物呈阳性，通过在768 bp处观察到特异性条带得以证实。在血液涂片结果呈阳性或阴性的疑似牛中均能检测到阳性结果，这证实了与传统诊断牛热带泰勒虫病的方法相比，PCR技术具有更高的灵敏度。PCR被证明是诊断牛热带泰勒虫病的一种高度灵敏和准确的方法，尤其是在检测血液和淋巴涂片呈阴性的样本时。
