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印度传统驱虫植物(L.)德拉布尔的体外杀绦虫活性。

In vitro cestocidal activity of (L.) Delarbre, a traditionally used anthelmintic plant in India.

作者信息

Nagi Keleni-I, Roy Bishnupada, Yadav Arun K

机构信息

Department of Zoology, North-Eastern Hill University, Shillong, 793022 India.

出版信息

J Parasit Dis. 2023 Mar;47(1):198-202. doi: 10.1007/s12639-022-01559-2. Epub 2022 Dec 5.

Abstract

is a medicinal plant used for the treatment of helminth infections among the Naga people of India. To verify the traditional claim of the plant, an in vitro anthelmintic efficacy of the methanol extract of leaves was investigated based on the paralytic and mortality effects on , an intestinal cestode parasite of domestic fowl, following exposure to 10, 20 and 30 mg/ml concentrations of extract. The effects of extract were also studied on the body surface of the parasites by scanning electron microscopy (SEM). The in vitro results showed an efficacy that was dose-dependent. At the highest dose (30 mg/ml), mortality of parasites occurred in 4.79 ± 0.17 h, in comparison to control, where the parasites survived till 45.63 ± 0.18 h. The SEM observations of extract-treated parasites revealed notable impairment in scolex, with distorted suckers and eroded spines. Also, the tegument was observed to be shrunken with impaired microtriches. The results indicate that leaves possess noteworthy anthelmintic efficacy and justify their use in traditional medicine against intestinal worms.

摘要

是一种药用植物,在印度那加人当中用于治疗蠕虫感染。为了验证该植物的传统药用价值,基于对家鸡肠道绦虫寄生虫暴露于10、20和30毫克/毫升浓度提取物后的麻痹和致死作用,研究了其叶子甲醇提取物的体外驱虫效果。还通过扫描电子显微镜(SEM)研究了提取物对寄生虫体表的影响。体外实验结果显示其效果具有剂量依赖性。在最高剂量(30毫克/毫升)下,寄生虫在4.79±0.17小时内死亡,而作为对照,寄生虫存活至45.63±0.18小时。对经提取物处理的寄生虫进行扫描电子显微镜观察发现,头节有明显损伤,吸盘扭曲,棘突侵蚀。此外,观察到皮层收缩,微毛受损。结果表明,该植物叶子具有显著的驱虫效果,证明其在传统医学中用于对抗肠道蠕虫是合理的。

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