University College of Pharmacy, University of the Punjab Lahore, Pakisatn.
University of the Punjab, University College of Pharmacy, University of the Punjab, Lahore, Pakistan.
Afr Health Sci. 2022 Sep;22(3):192-210. doi: 10.4314/ahs.v22i3.22.
Traditionally, was extensively used for the treatment of several human diseases.
This study was undertaken to determine in vivo effects of Berberis lyceum root bark against doxorubicin-induced cardiotoxicity and cisplatin-induced neurotoxicity in Sprague Dawley rats.
A single dose of doxorubicin (20 mg/ kg i. p) and cisplatin (4mg/kg i.p) was used to induce cardiotoxicity and neurotoxicity, respectively. Berberis lyceum methanolic extract was given orally (200 and 400 mg/ kg) to toxicity-induced rats. The cardiac biomarkers i.e. serum aspartate aminotransferase, alanine transaminase, lactate dehydrogenase, creatine kinase and creatine kinase MB were analyzed in blood collected from cardiotoxic rats. The tissue oxidative stress markers included protein, glutathione s-transferase specific activity, catalase activity, total glutathione, and malondialdehyde levels were measured in cardiac and brain homogenate of the respective groups.
Berberis lyceum methanolic extract has the potential to reduce the doxorubicin-induced cardiotoxicity and cisplatin-induced neurotoxicity significantly (*p<0.05) by reducing the serum markers and oxidative stress parameters. Histopathological analysis exhibited a marked improvement in the morphology of cardiac and brain tissues.
It is concluded that methanolic extract of Berberis lyceum root bark has the potential to protect and reverse anticancer drugs induced cardiotoxicity and neurotoxicity.
传统上，小檗碱被广泛用于治疗多种人类疾病。
本研究旨在确定贯叶连翘根皮对阿霉素诱导的心肌毒性和顺铂诱导的神经毒性的体内作用。
单次给予阿霉素（20mg/kg 腹腔注射）和顺铂（4mg/kg 腹腔注射）分别诱导心肌毒性和神经毒性。贯叶连翘甲醇提取物以 200 和 400mg/kg 剂量口服给予毒性诱导大鼠。从心肌毒性大鼠采集的血液中分析心脏生物标志物，即血清天冬氨酸氨基转移酶、丙氨酸转氨酶、乳酸脱氢酶、肌酸激酶和肌酸激酶同工酶。分别在各组心脏和脑组织匀浆中测量组织氧化应激标志物，包括蛋白质、谷胱甘肽 S-转移酶比活、过氧化氢酶活性、总谷胱甘肽和丙二醛水平。
贯叶连翘甲醇提取物通过降低血清标志物和氧化应激参数，具有显著降低阿霉素诱导的心肌毒性和顺铂诱导的神经毒性的潜力（*p<0.05）。组织病理学分析显示心脏和脑组织形态明显改善。
综上所述，贯叶连翘根皮的甲醇提取物具有保护和逆转抗癌药物诱导的心肌毒性和神经毒性的潜力。
