Islamic International Medical College, Rawalpindi, Pakistan.
Hazrat Bari Sarkar Medical College, Rawalpindi, Pakistan.
J Ayub Med Coll Abbottabad. 2021 Oct-Dec;33(4):553-557.
N acetyl cysteine and Berberis lycium Royale root bark have been used to treat kidney diseases. Objectives of the study were to evaluate the individual and combined effect of N acetyl cysteine and aqueous extract of Berberis lycium Royale root bark in Gentamicin induced nephrotoxicity in rats. This randomized control trial conducted at Islamic International Medical College, Rawalpindi in collaboration with NIH, Islamabad in 1 month from Sep to Oct 2020.
Fifty Wister albino rats of 10-12 weeks old were divided into five groups with 10 in each group. Group 1 was normal control given food and water only and remaining 40 were in treatment groups. Nephrotoxicity was induced by intraperitoneal injection of Gentamicin (80mg/kg) for 6 days in group 2, 3, 4 and 5. After induction of nephrotoxicity, Group 3 was administered N acetyl cysteine 140mg/kg per oral, Group 4 was given aqueous extract of Berberis lycium Royale root bark 400 mg/kg per oral and Group 5 was given both N acetyl cysteine 140mg/kg per oral and aqueous extract of Berberis lycium Royale root bark 400 mg/kg per oral for 21 days. Serum uric acid was measured in all groups after 30 days to observe the reversal of renal injury.
The results of this study indicate that Group 3, Group 4 and Group 5 showed a decrease in serum uric acid level as compared to Disease Control Group (Group 2). However, Group 5 significantly reduced uric acid (p-0.05).
Combined effect of N acetyl cysteine and aqueous extract of Berberis lycium Royale root bark showed improvement in uric acid level in Gentamicin induced nephrotoxicity in rats.
N-乙酰半胱氨酸和枸杞根皮已被用于治疗肾脏疾病。本研究的目的是评估 N-乙酰半胱氨酸和枸杞根皮水提物单独和联合使用对庆大霉素诱导的大鼠肾毒性的作用。这项随机对照试验于 2020 年 9 月至 10 月在伊斯兰堡 NIH 与拉瓦尔品第的伊斯兰国际医学院合作进行，为期 1 个月。
将 50 只 10-12 周龄的 Wistar 白化大鼠分为 5 组，每组 10 只。第 1 组为正常对照组，仅给予食物和水，其余 40 只为治疗组。第 2、3、4 和 5 组通过腹腔注射庆大霉素（80mg/kg）诱导肾毒性，共 6 天。在诱导肾毒性后，第 3 组给予 N-乙酰半胱氨酸 140mg/kg 口服，第 4 组给予枸杞根皮水提物 400mg/kg 口服，第 5 组给予 N-乙酰半胱氨酸 140mg/kg 口服和枸杞根皮水提物 400mg/kg 口服，共 21 天。在第 30 天测量所有组的血清尿酸，以观察肾损伤的逆转情况。
本研究结果表明，与疾病对照组（第 2 组）相比，第 3 组、第 4 组和第 5 组的血清尿酸水平降低。然而，第 5 组显著降低了尿酸（p-0.05）。
N-乙酰半胱氨酸和枸杞根皮水提物的联合作用可改善庆大霉素诱导的大鼠肾毒性中的尿酸水平。
