Zhang Chao, Feng Jinyan, Liu Yongheng, Zhang Yan, Song Weijie, Ma Yulin, Han Xiuxin, Wang Guowen
Department of Bone and Soft Tissue Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
Department of Animal Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
Front Oncol. 2023 Feb 22;13:1138837. doi: 10.3389/fonc.2023.1138837. eCollection 2023.
To explore the direct and indirect heat damage zone of radiofrequency ablation (RFA) in porcine vertebrae and to verify the safety of RFA in a vascularized vertebral tumor model.
RFA was performed in the porcine lumbar vertebrae. Magnetic resonance (MR) imaging, hematoxylin and eosin (HE), and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) were used to assess the extent of direct and indirect injuries after RFA. The cavity of lumbar vertebrae was made, and the adjacent muscle flap was used to fill the cavity to make a vertebrae tumor model. RFA was performed in the vascularized vertebral tumor model.
T1-weighted images showed a hypointensive region in the center surrounded by a more hypointensive rim on day 0 and 14. T2-weighted images showed that RFA zone was hypointensive on day 0. On day 7, hypointensity was detected in the center surrounded by a hyperintensive rim. HE showed that the RFA zone could be clearly observed on day 14. Thin bone marrow loss areas were seen around the RFA zone, which was consistent with the hyperintensive rim on the T2-weighted images. TUNEL showed a large number of apoptotic cells in the RFA zone. During RFA in the vertebral tumor model, the temperature of all monitoring positions was less than 45 °C.
Using experiments, the effective zone of RFA was evaluated by MR imaging and pathology, and the direct and indirect damage range were obtained. The safety of RFA was verified by RFA in a vascularized vertebral tumor model.
探讨射频消融（RFA）在猪椎体中的直接和间接热损伤区域，并验证RFA在血管化椎体肿瘤模型中的安全性。
在猪腰椎椎体中进行RFA。采用磁共振（MR）成像、苏木精-伊红（HE）染色和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法评估RFA后直接和间接损伤的程度。制作腰椎椎体空腔，并用相邻肌瓣填充空腔以制作椎体肿瘤模型。在血管化椎体肿瘤模型中进行RFA。
T1加权图像显示，在第0天和第14天，中心为低信号区，周围有更低信号的边缘。T2加权图像显示，RFA区域在第0天为低信号。在第7天，中心检测到低信号，周围有高信号边缘。HE染色显示，在第14天可清晰观察到RFA区域。在RFA区域周围可见薄的骨髓缺失区，这与T2加权图像上的高信号边缘一致。TUNEL显示RFA区域有大量凋亡细胞。在椎体肿瘤模型中进行RFA时，所有监测部位的温度均低于45℃。
通过实验，采用MR成像和病理学方法评估了RFA的有效区域，得出了直接和间接损伤范围。通过在血管化椎体肿瘤模型中进行RFA验证了其安全性。
