Genome-wide identification, expression profiling, and functional analysis of ammonium transporter 2 (AMT2) gene family in cassava ( crantz).

Nitrogen (N), absorbed primarily as ammonium (NH ) from soil by plant, is a necessary macronutrient in plant growth and development. Ammonium transporter (AMT) plays a vital role in the absorption and transport of ammonium (NH ). Cassava ( Crantz) has a strong adaptability to nitrogen deprivation. However, little is known about the functions of ammonium transporter AMT2 in cassava. The cassava AMT2-type genes were identified and their characteristics were analyzed using bioinformatic techniques. The spatial expression patterns were analyzed based on the public RNA-seq data and their expression profiles under low ammonium treatment were studied using Real-time quantitative PCR (RT-qPCR) method. The cassava genes were transformed into yeast mutant strain TM31019b by PEG/LiAc method to investigate their functions. Seven AMT2-type genes () were identified in cassava and they were distributed on 6 chromosomes and included two segmental duplication events ( and ). Based on their amino acid sequences, seven MeAMT2 were further divided into four subgroups, and each subgroup contained similar motif constitution and protein structure. Synteny analysis showed that two and four genes in cassava were collinear with those in the Arabidopsis and soybean genomes, respectively. Sixteen types of cis-elements were identified in the promoters, and they were related to light-, hormone-, stress-, and plant growth and development-responsive elements, respectively. Most of the genes displayed tissue-specific expression patterns according to the RNA-seq data, of them, three (, , and ) expressions were up-regulated under ammonium deficiency. Complementation experiments showed that yeast mutant strain TM31019b transformed with , , or grew better than untransgenic yeast cells under ammonium deficiency, suggesting that MeAMT2.3, MeAMT2.5, and MeATM2.6 might be the main contributors in response to ammonium deficiency in cassava. This study provides a basis for further study of nitrogen efficient utilization in cassava.