National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China.
Int J Mol Sci. 2022 Aug 23;23(17):9522. doi: 10.3390/ijms23179522.
Arbuscular mycorrhizal fungi (AMF) are important symbiotic microorganisms in soil that engage in symbiotic relationships with legumes, resulting in mycorrhizal symbiosis. Establishment of strong symbiotic relationships between AMF and legumes promotes the absorption of nitrogen by plants. Ammonium nitrogen can be directly utilised by plants following ammonium transport, but there are few reports on ammonium transporters (AMTs) promoting ammonium nitrogen transport during AM symbiosis. is a typical legume model plant that hosts AMF. In this study, we analysed the characteristics of the ammonium transporter , and found that it is a typical ammonium transporter with mycorrhizal-induced and ammonium nitrogen transport-related cis-acting elements in its promoter region. facilitated ammonium transfer in yeast mutant supplement experiments. In the presence of different nitrogen concentrations, the gene was significantly upregulated following inoculation with AMF, and induced by low nitrogen. Overexpression of increased the absorption of ammonium nitrogen, resulting in doubling of nitrogen content in leaves and roots, thus alleviating nitrogen stress and promoting plant growth.
丛枝菌根真菌（AMF）是土壤中重要的共生微生物，与豆科植物形成共生关系，称为菌根共生。AMF 与豆科植物之间建立强大的共生关系有助于植物吸收氮。植物可以直接利用铵态氮进行运输，但关于促进 AM 共生过程中铵态氮运输的铵转运体（AMTs）的报道较少。是一种典型的豆科模式植物，宿主 AMF。在这项研究中，我们分析了 的特征，发现它是一种典型的铵转运体，其启动子区域具有菌根诱导和铵态氮运输相关的顺式作用元件。在酵母突变体补充实验中促进了铵的转移。在不同氮浓度下，接种 AMF 后， 基因显著上调，并受低氮诱导。过表达 增加了对铵态氮的吸收，导致叶片和根系氮含量增加一倍，从而缓解氮胁迫，促进植物生长。
