Anwar Haseeb, Navaid Soha, Muzaffar Humaira, Hussain Ghulam, Faisal Muhammad Naeem, Ijaz Muhammad Umar, Riđanović Sanel
Department of Physiology Government College University Faisalabad Pakistan.
Institute of Physiology and Pharmacology University of Agriculture Faisalabad Punjab Pakistan.
Food Sci Nutr. 2023 Jan 11;11(3):1486-1498. doi: 10.1002/fsn3.3188. eCollection 2023 Mar.
The integrity of the distal alveolar epithelium is crucial for lung regeneration following an injury. The present study aimed to evaluate the effect of extract; cross-talk of epidermal growth factor () and erythropoietin () genes in a smoke-induced lung injury rat model. For experimentation ( = 27), albino rats were divided equally into three groups, i.e., negative control (NC), positive control (PC), and treatment group (TG). Cigarette smoke was exposed to PC and TG (4 CG/day). was given orally (350 mg/kg body weight) for 21 days. Decapitation ( = 3) was done on 14th, 18th, and 21st days, respectively. Analyses (hematology, biochemical, high performance liquid chromatography [HPLC], histology, and gene expression) were carried out and results were statistically analyzed by two-way analysis of variance. HPLC analysis of ethanolic extract of was done to identify the presence of phenolic constituents which showed high concentrations of quercetin and P-coumaric acid. Serum oxidative parameters such as total oxidant status, malondialdehyde, and hematological parameters such as red blood cells, hemoglobin, hematocrit, and white blood cells were significantly ( < .05) elevated in the PC group; however, these parameters were significantly ( < .05) improved in TG. While total antioxidant capacity and serum parameters such as total protein, albumin, and globulin were significantly ( < .05) reduced in the PC group but significantly improved ( < .05) in TG. Histological analysis revealed that smoke exposure resulted in a measurable increase in alveolar septal thickening while ethanolic extract of greatly ameliorated the histopathological changes in the lung alveoli. The gene expression analysis of and genes showed a significant upregulation ( < .05) of both genes in PC group while in TG, the level of both genes downregulated, in which lung damage was ameliorated due to cytoprotective effects of ethanolic extract of
远端肺泡上皮的完整性对于损伤后的肺再生至关重要。本研究旨在评估提取物的作用；在烟雾诱导的肺损伤大鼠模型中表皮生长因子（）和促红细胞生成素（）基因的相互作用。为了进行实验（ = 27），将白化大鼠平均分为三组，即阴性对照组（NC）、阳性对照组（PC）和治疗组（TG）。对PC组和TG组暴露香烟烟雾（每天4支香烟）。口服给予（350毫克/千克体重），持续21天。分别在第14天、第18天和第21天进行断头处死（ = 3）。进行了分析（血液学、生化、高效液相色谱[HPLC]、组织学和基因表达），并通过双向方差分析对结果进行了统计分析。对的乙醇提取物进行HPLC分析，以确定酚类成分的存在，结果显示槲皮素和对香豆酸浓度较高。PC组中血清氧化参数如总氧化剂状态、丙二醛以及血液学参数如红细胞、血红蛋白、血细胞比容和白细胞均显著（ < 0.05）升高；然而，这些参数在TG组中显著（ < 0.05）改善。虽然PC组中总抗氧化能力以及血清参数如总蛋白、白蛋白和球蛋白显著（ < 0.05）降低，但在TG组中显著改善（ < 0.05）。组织学分析显示，烟雾暴露导致肺泡间隔增厚有可测量增加，而的乙醇提取物极大地改善了肺泡的组织病理学变化。和基因的基因表达分析显示，PC组中这两个基因均显著上调（ < 0.05），而在TG组中，这两个基因的水平下调，其中由于的乙醇提取物的细胞保护作用，肺损伤得到改善
