Clin Lab. 2023 Mar 1;69(3). doi: 10.7754/Clin.Lab.2022.220536.
Urine osmolality determines the concentration ability of the kidney. Therefore, it is used to assess the body's hydration status, electrolyte levels, and acid-base disturbances. We aimed to evaluate the analytical performance of osmolality measurement of the Sysmex UF-5000 (UF-5000), to examine the effect of different molecules and particles in the urine on the osmolality measurement.
Complete urinalysis and conductivity-based osmolality analysis with UF-5000 and osmolality analysis with Advanced® Model 3320 Micro-Osmometer (AI-3320) by freezing point reduction method were performed in the urine samples. Samples were grouped as negative, glucosuria, proteinuria, hematuria, pyuria, crystalluria, and urobilinogen.
Total impressions were calculated as < 5% and accuracy values were < 1.66% in both analyzers. The regression equation was found to be y = -12.54 + 0.956x and the relative difference between the analyzers was 8.7% in 586 samples. When patients with Glucose > 2+ were excluded the regression equation of 507 samples was found as y = 5.10 + 0.948x and the relative difference was 4.6%. The percentages of samples with a difference greater than the allowable difference were 18.8%, 11.6%, 35.9%, 13.7%, 18.7%, and < 12.2% in all samples, all samples without glucosuria > 2+, glucosuria, glucosuria < 3+, proteinuria, and other subgroups, respectively.
Considering the good accessibility of the automated routine complete urine analyzer, UF-5000 can be considered to determine whether urine osmolality is within reference or should be measured by methods based on colligative properties. Thus, referral of patients to a clinic that uses the colligative measurement method may be used more effectively.
尿渗透压决定了肾脏的浓缩能力。因此，它被用于评估人体的水合状态、电解质水平和酸碱平衡紊乱。我们旨在评估 Sysmex UF-5000（UF-5000）的渗透压测量的分析性能，并研究不同尿液中的分子和颗粒对渗透压测量的影响。
对尿液样本进行完整的尿液分析和基于 UF-5000 的电导率渗透压分析以及基于冰点降低法的 Advanced® Model 3320 微渗透压计（AI-3320）的渗透压分析。样本被分为阴性、糖尿、蛋白尿、血尿、脓尿、结晶尿和尿胆原。
两个分析仪的总印象值均计算为<5%，准确度值均<1.66%。在 586 个样本中，发现回归方程为 y = -12.54 + 0.956x，两个分析仪之间的相对差异为 8.7%。当排除葡萄糖>2+的患者后，发现 507 个样本的回归方程为 y = 5.10 + 0.948x，相对差异为 4.6%。在所有样本、所有无葡萄糖>2+的样本、葡萄糖<3+的糖尿样本、蛋白尿样本和其他亚组中，差异大于允许差异的样本百分比分别为 18.8%、11.6%、35.9%、13.7%、18.7%和<12.2%。
考虑到自动化常规完整尿液分析仪的良好可及性，UF-5000 可用于确定尿液渗透压是否在参考范围内，或者是否应通过基于依数性的方法进行测量。因此，将患者转介到使用依数性测量方法的诊所可能会更有效地进行。
