Clin Lab. 2023 Mar 1;69(3). doi: 10.7754/Clin.Lab.2022.220614.
Alzheimer's disease (AD) is the most common form of dementia. In this study, serum levels of autophagy-related 5 (ATG5), apolipoprotein B-48, thyroid hormones, and homocysteine were examined in patients with AD to determine their diagnostic and predictive value for early diagnosis and prevention of AD.
For this study, fifty serum samples were obtained from patients with AD and fifty serum samples from healthy controls. Serum levels of ATG 5, apo B48, thyroid hormones, homocysteine, vitamin B12, and folic acid were determined by ELISA. Spectrophotometry was used to determine serum lipid concentrations.
The mean age of the case group was 69 ± 6.4 years and that of the control group was 67 ± 4.2 years. There were differences between the control and case groups in serum levels of homocysteine, apo B48, ATG5, hsCRP, LDL, HDL, cholesterol, and VitB12 (p < 0.05). According to the results of the ROC curve, measurements of serum levels of ATG5, homocysteine, and apo B48 have excellent performance in distinguishing patients with Alzheimer's disease from patients without AD.
This study suggests that the measurement of serum levels of ATG5, homocysteine, and apo B48, along with other available biomarkers, can be helpful in the diagnosis and management of patients with AD in the early stages of their disease.
阿尔茨海默病（AD）是最常见的痴呆症形式。在这项研究中，检查了 AD 患者的自噬相关蛋白 5（ATG5）、载脂蛋白 B-48、甲状腺激素和同型半胱氨酸的血清水平，以确定它们对早期诊断和预防 AD 的诊断和预测价值。
为此研究，从 AD 患者和健康对照者中各获得了 50 份血清样本。通过 ELISA 测定血清中 ATG5、apo B48、甲状腺激素、同型半胱氨酸、维生素 B12 和叶酸的水平。使用分光光度法测定血清脂质浓度。
病例组的平均年龄为 69 ± 6.4 岁，对照组为 67 ± 4.2 岁。病例组和对照组之间在同型半胱氨酸、apo B48、ATG5、hsCRP、LDL、HDL、胆固醇和 VitB12 的血清水平上存在差异（p < 0.05）。根据 ROC 曲线的结果，测量血清中 ATG5、同型半胱氨酸和 apo B48 的水平在区分阿尔茨海默病患者和无 AD 的患者方面表现出色。
本研究表明，测量血清中 ATG5、同型半胱氨酸和 apo B48 的水平，以及其他可用的生物标志物，有助于在疾病的早期阶段诊断和管理 AD 患者。
