Department of Developmental Biology and Cancer Research, Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School.
Department of Developmental Biology and Cancer Research, Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School; Department of Military Medicine and "Tzameret", Faculty of Medicine, Hebrew University of Jerusalem, and Medical Corps, Israel Defense Forces.
J Vis Exp. 2023 Feb 24(192). doi: 10.3791/64743.
Mice are a common model organism used to study metabolic diseases such as diabetes mellitus. Glucose levels are typically measured by tail-bleeding, which requires handling the mice, causes stress, and does not provide data on freely behaving mice during the dark cycle. State-of-the-art continuous glucose measurement in mice requires inserting a probe into the aortic arch of the mouse, as well as a specialized telemetry system. This challenging and expensive method has not been adopted by most labs. Here, we present a simple protocol involving the utilization of commercially available continuous glucose monitors used by millions of patients to measure glucose continuously in mice as a part of basic research. The glucose-sensing probe is inserted into the subcutaneous space in the back of the mouse through a small incision to the skin and is held in place tightly using a couple of sutures. The device is sutured to the mouse skin to ensure it remains in place. The device can measure glucose levels for up to 2 weeks and sends the data to a nearby receiver without any need to handle the mice. Scripts for the basic data analysis of glucose levels recorded are provided. This method, from surgery to computational analysis, is cost-effective and potentially very useful in metabolic research.
小鼠是用于研究代谢性疾病（如糖尿病）的常见模式生物。通常通过尾部采血来测量血糖水平，这需要处理小鼠，会造成应激，并且无法提供暗周期中自由活动的小鼠的数据。目前最先进的小鼠连续血糖测量需要将探头插入小鼠的主动脉弓，并使用专门的遥测系统。这种具有挑战性和昂贵的方法尚未被大多数实验室采用。在这里，我们提出了一种简单的方案，涉及使用商业上可用于连续监测血糖的监测器，数百万患者都使用这些监测器来进行基础研究中连续监测小鼠的血糖。将葡萄糖感应探头通过皮肤的小切口插入小鼠背部的皮下空间，并使用几针缝线将其固定在位。将设备缝合到小鼠皮肤上，以确保其保持在位。该设备可以测量长达 2 周的血糖水平，并且无需处理小鼠即可将数据发送到附近的接收器。还提供了用于记录的血糖水平的基本数据分析脚本。从手术到计算分析，这种方法具有成本效益，在代谢研究中可能非常有用。
