Department of Diabetes, School of Life Course Sciences, King's College London, London, UK.
School of Cancer & Pharmaceutical Sciences, King's College London, London, UK.
Methods Mol Biol. 2020;2128:225-239. doi: 10.1007/978-1-0716-0385-7_16.
Measurement of blood glucose concentration is a common end point in studies using animal models of diabetes. Usually a blood glucose meter is used to measure non-fasted blood glucose concentrations, typically at frequencies of between 1 and 7 times per week. This process involves pricking the tip of the tail to collect a small blood sample (0.5-5 μL), which could potentially cause a stress response and affect blood glucose concentrations. Moreover, with blood glucose concentrations constantly fluctuating in response to feeding and activity, a single-point measurement can easily misrepresent the actual glycemic control of the animal. In this chapter, we discuss the use of continuous glucose monitoring in mice by radio-telemetry which allows second-by-second changes in blood glucose to be captured without restraining the mouse. Glucose excursions rather than single-point measurements may prove more useful in detecting effects of treatments, and lack of handling may avoid stress responses causing artefacts. We outline what is involved in implanting such devices into mice including some practical tips to maximize success.
血糖浓度的测量是使用糖尿病动物模型进行研究的常见终点。通常使用血糖仪测量非禁食状态下的血糖浓度，通常每周测量 1 到 7 次。这一过程涉及刺破尾巴尖以采集一小份血样（0.5-5 μL），这可能会引起应激反应并影响血糖浓度。此外，由于血糖浓度会随着进食和活动而不断波动，单点测量很容易无法真实反映动物的实际血糖控制情况。在本章中，我们将讨论通过无线电遥测术在小鼠中使用连续血糖监测，这可以在不限制小鼠的情况下捕获血糖的逐秒变化。与单点测量相比，血糖波动可能更有助于发现治疗效果，并且减少操作可以避免引起人为假象的应激反应。我们概述了将这些设备植入小鼠体内的相关操作，包括一些最大化成功的实用技巧。
