Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA.
Henan Agricultural University, Zhengzhou, 450002, Henan Province, China.
Theor Appl Genet. 2023 Mar 13;136(3):52. doi: 10.1007/s00122-023-04284-3.
Two QTLs with major effects on rolled leaf trait were consistently detected on chromosomes 1A (QRl.hwwg-1AS) and 5A (QRl.hwwg-5AL) in the field experiments. Rolled leaf (RL) is a morphological strategy to protect plants from dehydration under stressed field conditions. Identification of quantitative trait loci (QTLs) underlining RL is essential to breed drought-tolerant wheat cultivars. A mapping population of 154 recombinant inbred lines was developed from the cross between JagMut1095, a mutant of Jagger, and Jagger to identify quantitative trait loci (QTLs) for the RL trait. A linkage map of 3106 cM was constructed with 1003 unique SNPs from 21 wheat chromosomes. Two consistent QTLs were identified for RL on chromosomes 1A (QRl.hwwg-1AS) and 5A (QRl.hwwg-5AL) in all field experiments. QRl.hwwg-1AS explained 24-56% of the phenotypic variation and QRl.hwwg-5AL explained up to 20% of the phenotypic variation. The combined percent phenotypic variation associated with the two QTLs was up to 61%. Analyses of phenotypic and genotypic data of recombinants generated from heterogeneous inbred families of JagMut1095 × Jagger delimited QRl.hwwg-1AS to a 6.04 Mb physical interval. This work lays solid foundation for further fine mapping and map-based cloning of QRl.hwwg-1AS.
两个主效 QTL 被一致检测到在染色体 1A（QRl.hwwg-1AS）和 5A（QRl.hwwg-5AL）上控制卷曲叶性状。卷曲叶（RL）是一种植物在田间胁迫条件下防止脱水的形态策略。鉴定 RL 相关的数量性状位点（QTL）对于培育耐旱小麦品种至关重要。JagMut1095 是 Jagger 的一个突变体，通过 JagMut1095 和 Jagger 的杂交，产生了一个包含 154 个重组自交系的作图群体，用于鉴定 RL 性状的数量性状位点（QTL）。利用来自 21 条小麦染色体的 1003 个独特 SNP，构建了一个 3106cM 的连锁图谱。在所有田间试验中，在染色体 1A（QRl.hwwg-1AS）和 5A（QRl.hwwg-5AL）上一致鉴定到两个控制 RL 的 QTL。QRl.hwwg-1AS 解释了 24-56%的表型变异，QRl.hwwg-5AL 解释了高达 20%的表型变异。两个 QTL 共同解释了高达 61%的表型变异。对来自 JagMut1095×Jagger 的异质自交系群体的重组体的表型和基因型数据的分析，将 QRl.hwwg-1AS 限定在一个 6.04Mb 的物理区间内。这项工作为进一步精细定位和基于图谱的克隆 QRl.hwwg-1AS 奠定了坚实的基础。
