State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Theor Appl Genet. 2012 Jan;124(1):177-88. doi: 10.1007/s00122-011-1696-3. Epub 2011 Sep 30.
Wheat yellow mosaic (WYM) caused by wheat yellow mosaic bymovirus (WYMV) has been growing as one of the most serious diseases affecting wheat production in China. In this study, the association of quantitative trait loci (QTLs) governing WYMV resistance with molecular markers was established using 164 recombinant inbred lines (RILs) derived from 'Xifeng Wheat' (highly resistant) × 'Zhen 9523' (highly susceptible). Phenotypic data of WYMV resistance of the RILs were collected from 4-year, two-location replicated field trials. A molecular marker-based linkage map, which was comprised of 273 non-redundant loci and represented all the 21 wheat chromosomes, was constructed with the JoinMap 4.0 software. Using the Windows QTL Cartographer V2.5 software, three QTLs associated with WYMV resistance, QYm.njau-3B.1, QYm.njau-5A.1 and QYm.njau-7B.1, were detected on chromosomes 3BS, 5AL, and 7BS, respectively. The favorable allele effects were all contributed by 'Xifeng Wheat'. Among the three QTLs, QYm.njau-3B.1 and QYm.njau-5A.1 were detected in all the four trials and the overall mean, and could explain 3.3-10.2% and 25.9-53.7% of the phenotypic variation, respectively, while QYm.njau-7B.1 was detected in one trial and the overall mean and explained 4.9 and 3.3% of the phenotypic variation, respectively. A large portion of the variability for WYMV response was explained by a major QTL, QYm.njau-5A.1. The relationship of the molecular markers linked with QYm.njau-5A.1 and the WYMV resistance was further validated using a secondary F(2) population. The results showed that three markers, i.e., Xwmc415.1, CINAU152, and CINAU153, were closely linked to QYm.njau-5A.1 with the genetic distances of 0.0, 0.0, and 0.1 cM, respectively, indicating they should be useful in marker-assisted selection (MAS) wheat breeding for WYMV resistance. A panel of germplasm collection consisting of 46 wheat varieties with known WYMV response phenotypes was further used to validate the presence and effects of QYm.njau-5A.1 and the above three markers. It was found that QYm.njau-5A.1 was present in 12 of the 34 WYMV-resistant varieties.
小麦黄花叶病（WYM）是由小麦黄花叶病毒（WYMV）引起的，是中国小麦生产中最严重的病害之一。本研究利用“西峰小麦”（高抗）ד郑 9523”（高感）衍生的 164 个重组自交系（RILs），建立了与抗 WYMV 数量性状基因座（QTLs）相关的分子标记关联。通过 4 年、2 个地点的田间重复试验，收集了 RILs 对 WYMV 的抗性表型数据。使用 JoinMap 4.0 软件构建了一个基于分子标记的连锁图谱，该图谱由 273 个非冗余位点组成，代表了 21 条小麦染色体。利用 Windows QTL Cartographer V2.5 软件，在 3BS、5AL 和 7BS 染色体上分别检测到与 WYMV 抗性相关的 3 个 QTL，分别命名为 QYm.njau-3B.1、QY m.njau-5A.1 和 QYm.njau-7B.1。有利等位基因效应均来自“西峰小麦”。在这 3 个 QTL 中，QY m.njau-3B.1 和 QYm.njau-5A.1在所有 4 次试验和总平均值中均被检测到，分别可解释 3.3-10.2%和 25.9-53.7%的表型变异，而 QYm.njau-7B.1仅在一次试验和总平均值中被检测到，分别解释了 4.9%和 3.3%的表型变异。WYMV 反应的大部分变异性由一个主效 QTL，QY m.njau-5A.1 解释。利用二次 F2 群体进一步验证了与 QYm.njau-5A.1 相关的分子标记与 WYMV 抗性的关系。结果表明，与 QYm.njau-5A.1 紧密连锁的 3 个标记 Xwmc415.1、CINAU152 和 CINAU153 的遗传距离分别为 0.0、0.0 和 0.1cM，表明它们在 WYMV 抗性的 MAS 小麦育种中应该是有用的。进一步利用包含 46 个已知 WYMV 反应表型的小麦种质资源的小组，验证了 QYm.njau-5A.1 和上述 3 个标记的存在和效应。结果发现，在 34 个抗 WYMV 的品种中，有 12 个品种存在 QYm.njau-5A.1。
