NIR Mitochondrial Fluorescent Probe for Visualizing SO/Polarity in Drug Induced Inflammatory Mice.

SO and polarity are important microenvironmental parameters in cells, which are closely related to physiological activities in organisms. The intracellular levels of SO and polarity are abnormal in inflammatory models. To this end, a novel near-infrared fluorescent probe BTHP that can simultaneously detect SO and polarity was studied. BTHP can sensitively detect polarity change with emission peak change from 677 to 818 nm. BTHP can also detect SO with fluorescence change from red to green. After addition of SO, the fluorescence emission intensity ratio / of the probe increased by about 33.6 times. BTHP can determine bisulfite in single crystal rock sugar with high recovery rate (99.2%-101.7%). Fluorescence imaging of cells showed that BTHP could better target mitochondria and monitor exogenous SO in A549 cells. More importantly, BTHP has been successfully used for dual channel monitoring SO and polarity in drug-induced inflammatory cells and mice. In particular, the probe showed increased green fluorescence with the generation of SO and increased red fluorescence with the decrease of polarity in inflammatory cells and mice.