Moin Nida, Thakur Ravindra Singh, Singh Swati, Patel Devendra Kumar, Satish Aruna
Ecotoxicology Laboratory, Environmental Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhavan, 31, Mahatma Gandhi Marg, Lucknow, 226001, Uttar Pradesh, India; Department of Biochemistry, Babu Banarasi Das University, Lucknow, 227015, India.
Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhavan, 31, Mahatma Gandhi Marg, Lucknow, 226001, India.
Chemosphere. 2023 Jun;326:138353. doi: 10.1016/j.chemosphere.2023.138353. Epub 2023 Mar 11.
β-triketone herbicides have been efficiently employed as an alternate to atrazine. Triketones are 4-hydroxyphenylpyruvate dioxygenase (HPPD) enzyme inhibitors and exposure is reported to cause significant increase in plasma tyrosine levels. In this study, we have employed a non-target organism Caenorhabditis elegans to determine the impact of β-triketone exposures at recommended field doses (RfD). Our results indicate sulcotrione and mesotrione, negatively influence the survival, behavior, and reproduction of the organism at RfD. Additionally, we have traced the parallels regarding the impact of triketones on the tyrosine metabolism pathway, in C. elegans to those in mammalian models, wherein the expression of the tyrosine metabolism pathway genes are altered, directly influencing tyrosine catabolism leading to significant tyrosine accumulation in exposed organism. Further, we investigated the impact of sulcotrione and mesotrione exposure on fat deposition (triglyceride levels, Oil-Red-O staining and lipidomics) and the fatty acid metabolism pathway. In the exposed worms, the expression of enlongases and fatty acid desaturases were up-regulated along with an increase in the levels of triglycerides. Thus, the data indicates a positive association of β-triketone exposure to mis-regulation of the fatty acid metabolism pathway genes leading to fat accumulation in worms. Therefore, β-triketone might be a potential obesogen.
β-三酮类除草剂已被有效地用作阿特拉津的替代品。三酮类是4-羟基苯丙酮酸双加氧酶（HPPD）的酶抑制剂，据报道，接触此类物质会导致血浆酪氨酸水平显著升高。在本研究中，我们使用了非靶标生物秀丽隐杆线虫，以确定在推荐田间剂量（RfD）下接触β-三酮类物质的影响。我们的结果表明，在RfD剂量下，磺草酮和甲基磺草酮会对该生物体的生存、行为和繁殖产生负面影响。此外，我们还追踪了三酮类物质对秀丽隐杆线虫酪氨酸代谢途径的影响与对哺乳动物模型影响之间的相似之处，其中酪氨酸代谢途径基因的表达发生改变，直接影响酪氨酸分解代谢，导致受暴露生物体中酪氨酸大量积累。此外，我们研究了磺草酮和甲基磺草酮暴露对脂肪沉积（甘油三酯水平、油红O染色和脂质组学）以及脂肪酸代谢途径的影响。在受暴露的线虫中，延长酶和脂肪酸去饱和酶的表达上调，同时甘油三酯水平增加。因此，数据表明β-三酮类物质暴露与脂肪酸代谢途径基因的失调呈正相关，导致线虫体内脂肪积累。所以，β-三酮类物质可能是一种潜在的致肥胖物。
