School of Food and Advanced Technology, Massey University, Private Bag 11222, Palmerston North, New Zealand.
The New Zealand Institute for Plant & Food Research Limited, Private Bag 92169, Auckland 1142, New Zealand.
Food Res Int. 2023 Apr;166:112605. doi: 10.1016/j.foodres.2023.112605. Epub 2023 Feb 16.
Vibrio parahaemolyticus biofilms on the seafood processing plant surfaces are a potential source of seafood contamination and subsequent food poisoning. Strains differ in their ability to form biofilm, but little is known about the genetic characteristics responsible for biofilm development. In this study, pangenome and comparative genome analysis of V. parahaemolyticus strains reveals genetic attributes and gene repertoire that contribute to robust biofilm formation. The study identified 136 accessory genes that were exclusively present in strong biofilm forming strains and these were functionally assigned to the Gene Ontology (GO) pathways of cellulose biosynthesis, rhamnose metabolic and catabolic processes, UDP-glucose processes and O antigen biosynthesis (p < 0.05). Strategies of CRISPR-Cas defence and MSHA pilus-led attachment were implicated via Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation. Higher levels of horizontal gene transfer (HGT) were inferred to confer more putatively novel properties on biofilm-forming V. parahaemolyticus. Furthermore, cellulose biosynthesis, a neglected potential virulence factor, was identified as being acquired from within the order Vibrionales. The cellulose synthase operons in V. parahaemolyticus were examined for their prevalence (22/138, 15.94 %) and were found to consist of the genes bcsG, bcsE, bcsQ, bcsA, bcsB, bcsZ, bcsC. This study provides insights into robust biofilm formation of V. parahaemolyticus at the genomic level and facilitates: identification of key attributes for robust biofilm formation, elucidation of biofilm formation mechanisms and development of potential targets for novel control strategies of persistent V. parahaemolyticus.
副溶血性弧菌在海鲜加工厂表面形成的生物膜是海鲜污染和随后食物中毒的潜在来源。菌株在形成生物膜的能力上存在差异，但对于导致生物膜形成的遗传特征知之甚少。在这项研究中，对副溶血性弧菌菌株的泛基因组和比较基因组分析揭示了有助于形成强生物膜的遗传特征和基因库。研究确定了 136 个 accessory 基因，这些基因仅存在于强生物膜形成菌株中，这些基因被功能分配到纤维素生物合成、鼠李糖代谢和分解代谢过程、UDP-葡萄糖过程和 O 抗原生物合成的基因本体论 (GO) 途径（p<0.05）。京都基因与基因组百科全书 (KEGG) 注释表明涉及 CRISPR-Cas 防御和 MSHA 菌毛介导的附着策略。通过水平基因转移 (HGT) 的推断，赋予了生物膜形成副溶血性弧菌更多推测的新特性。此外，纤维素生物合成，一个被忽视的潜在毒力因子，被确定是从 Vibrionales 目中获得的。研究了副溶血性弧菌中纤维素合酶操纵子的普遍性（22/138，15.94%），并发现它们由 bcsG、bcsE、bcsQ、bcsA、bcsB、bcsZ 和 bcsC 基因组成。这项研究提供了在基因组水平上对副溶血性弧菌强生物膜形成的深入了解，并有助于：确定强生物膜形成的关键属性，阐明生物膜形成机制，并开发针对持久副溶血性弧菌的新型控制策略的潜在目标。
