Mailhes J B, Yuan Z P
Department of Obstetrics and Gynecology, Louisiana State University Medical Center, Shreveport 71130.
Environ Mol Mutagen. 1987;10(2):183-8. doi: 10.1002/em.2850100208.
Unpublished results from our laboratory showed that colchicine increased the incidence of hyperploid mouse metaphase II (MII) oocytes when injected at the same time as human chorionic gonadotrophin (HCG). The objective of the present study was to determine whether the time of administering colchicine influenced the incidence of aneuploidy in MII oocytes. CD-1 mice were given pregnant mare's serum (PMS) and 48 hr later, HCG. An intraperitoneal injection of 0.2 mg/kg colchicine was given at +4, +2, 0, -2, or -4 hr relative to HCG. Oocytes were collected 17 hr post-HCG and processed, and chromosomes were subsequently C-banded. The percentage of hyperploid oocytes was 0.77, 2.56, 5.71, 7.79, 3.54, and 2.70 for control, +4, +2, 0, -2, or -4 hr pre/post-HCG, respectively. Chi-square analyses of these data demonstrated that colchicine significantly increases the proportion of aneuploid oocytes, and that the relative sensitivity of colchicine-induced aneuploidy depends upon the time that this drug is administered relative to HCG.
我们实验室未发表的结果表明，秋水仙碱与人绒毛膜促性腺激素（HCG）同时注射时，会增加超倍体小鼠中期II（MII）卵母细胞的发生率。本研究的目的是确定秋水仙碱的给药时间是否会影响MII卵母细胞中非整倍体的发生率。给CD-1小鼠注射孕马血清（PMS），48小时后注射HCG。相对于HCG，在+4、+2、0、-2或-4小时腹腔注射0.2mg/kg秋水仙碱。在注射HCG后17小时收集卵母细胞并进行处理，随后对染色体进行C带染色。对照组、HCG前/后+4、+2、0、-2或-4小时的超倍体卵母细胞百分比分别为0.77%、2.56%、5.71%、7.79%、3.54%和2.70%。对这些数据进行卡方分析表明，秋水仙碱显著增加了非整倍体卵母细胞的比例，并且秋水仙碱诱导非整倍体的相对敏感性取决于该药物相对于HCG的给药时间。
