Mailhes J B, Young D, London S N
Department of Obstetrics and Gynecology, Louisiana State University Medical Center, Shreveport 71130, USA.
Biol Reprod. 1997 Jul;57(1):92-8. doi: 10.1095/biolreprod57.1.92.
Aneuploidy in germ cells results in reproductive failure and mental and physical disorders in humans. Unfortunately, little is known about the causes and mechanisms of aneuploidy induction. The objective of this study was to test the hypothesis that propylene glycol (1,2-propanediol; PG) induces cytogenetic aberrations in mouse metaphase II (MII) oocytes that predispose zygotes to aneuploidy. Female ICR mice received 7.5 IU eCG and 5.0 IU hCG 48 h later. PG doses of 1300, 2600, and 5200 mg/kg body weight were given 3 h post-hCG; controls received the solvent deionized water. Ovulated oocytes were collected 16 h after administration of PG and processed for cytogenetic analysis. For the one-cell zygote cytogenetic study, females were given PG and paired (1:1) with ICR males for 16 h. Females that mated were given 2 x 10(-3) M colchicine 22 h post-PG, and zygotes were collected 18 h later. PG significantly (p < 0.05) increased both the proportion of MII oocytes with premature centromere separation (PCS) and the proportion of aneuploid one-cell zygotes. These results support the hypothesis that PG-induced PCS in MII oocytes predisposes zygotes to aneuploidy.
生殖细胞中的非整倍体导致人类生殖失败以及精神和身体疾病。不幸的是，关于非整倍体诱导的原因和机制知之甚少。本研究的目的是检验以下假设：丙二醇（1,2 - 丙二醇；PG）会诱导小鼠中期II（MII）卵母细胞出现细胞遗传学畸变，使受精卵易发生非整倍体。雌性ICR小鼠先接受7.5 IU的促卵泡素（eCG），48小时后再接受5.0 IU的人绒毛膜促性腺激素（hCG）。在注射hCG后3小时给予PG，剂量分别为1300、2600和5200毫克/千克体重；对照组接受溶剂去离子水。在给予PG 16小时后收集排卵的卵母细胞并进行细胞遗传学分析。对于单细胞受精卵细胞遗传学研究，给雌性小鼠注射PG并与ICR雄性小鼠按1:1配对16小时。交配后的雌性小鼠在注射PG后22小时给予2×10⁻³ M的秋水仙碱，18小时后收集受精卵。PG显著（p < 0.05）增加了具有早发性着丝粒分离（PCS）的MII卵母细胞比例以及非整倍体单细胞受精卵的比例。这些结果支持了以下假设：PG诱导MII卵母细胞出现PCS会使受精卵易发生非整倍体。
