针刺治疗的哮喘大鼠血清可调节气道平滑肌细胞中p38丝裂原活化蛋白激酶（p38-MAPK）的激活。
Serum from acupuncture-treated asthmatic rats regulates p38-MAPK activation in airway smooth muscle cells.
作者信息
Yu Yueyue, Cui Jianmei, Sun Na, Liu Huijuan, Wang Hongbin, Yang Jinhua
机构信息
Department of Traditional Chinese Medicine, North China University of Science and Technology 21 Bohai Road, Caofeidian Xincheng, Tangshan 063210, Hebei, China.
出版信息
Am J Transl Res. 2023 Feb 15;15(2):1063-1071. eCollection 2023.
OBJECTIVES
This study aimed to investigate the regulation function of acupuncture on airway smooth muscle cells (ASMCs) of asthmatic rats.
METHODS
Male Sprague-Dawley rats were challenged using inhalational Ovalbumin (OVA) to establish an asthma model. The acupuncture points (GV14 for Dazhui, bilateral BL12 for Fengmen, and bilateral BL13 for Feishu) were stimulated for asthma relief. The ASMCs isolated from asthmatic rats were incubated in medium containing the serum obtained from asthmatic rats treated with acupuncture. The expression levels of p38 MAPK and p-p38 MAPK were determined by immunocytochemical and western blot.
RESULTS
ASMCs were successfully isolated and cultured. The 20% acupuncture treatment of asthmatic rat serum had the least effect on the proliferation ability of asthmatic ASMCs. The serum from asthmatic rats treated with acupuncture could decrease the expression of p-p38 MAPK in asthmatic rat ASMCs.
CONCLUSIONS
The serum from acupuncture-treated asthmatic rats has an effect on treating asthma in rats, and the mechanism of action may be by regulating the p38 pathway.
目的
本研究旨在探讨针刺对哮喘大鼠气道平滑肌细胞（ASMCs）的调节作用。
方法
采用雄性Sprague-Dawley大鼠，通过吸入卵清蛋白（OVA）建立哮喘模型。针刺大椎（GV14）、双侧风门（BL12）和双侧肺俞（BL13）穴位以缓解哮喘。将从哮喘大鼠分离的ASMCs培养于含有经针刺治疗的哮喘大鼠血清的培养基中。通过免疫细胞化学和蛋白质印迹法测定p38丝裂原活化蛋白激酶（p38 MAPK）和磷酸化p38丝裂原活化蛋白激酶（p-p38 MAPK）的表达水平。
结果
成功分离并培养了ASMCs。20%针刺治疗的哮喘大鼠血清对哮喘ASMCs增殖能力影响最小。针刺治疗的哮喘大鼠血清可降低哮喘大鼠ASMCs中p-p38 MAPK的表达。
结论
针刺治疗的哮喘大鼠血清对大鼠哮喘具有治疗作用，其作用机制可能是通过调节p38信号通路。
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