Bakr Amira Abo, Ali Mohamed, Ibrahim Khairy
Biochemistry Division, Faculty of Science, Cairo University, Egypt.
Mammalian Toxicology Department, Central Agricultural Pesticides Laboratory, Agricultural Research Center, Dokki, Giza, 12618, Egypt.
Environ Anal Health Toxicol. 2022 Dec;37(4):e2022037-0. doi: 10.5620/eaht.2022037. Epub 2022 Nov 23.
Fipronil can cause oxidative tissue damage and apoptosis. Our goal is to evaluate the antiapoptotic impact of garlic or allopurinol against fipronil neurotoxicity. Thirty-six mature male albino rats were separated into control, garlic aqueous extract (500 mg/kg), allopurinol (150 mg/L in their drinking water), fipronil (13.277 mg/kg), garlic+fipronil, and allopurinol+fipronil. Our results revealed that fipronil induced a significant increase in brain malondialdehyde, protein carbonyl levels as well as enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione peroxidase, and xanthine oxidase), but glutathione-S-transferase recorded a significant decrease as compared to the control. In addition, fipronil significantly up-regulated the brain pro-apoptotic (Bax) and caspase -3 mRNA gene expression and induced DNA fragmentation but caused down-regulation in anti-apoptotic (Bcl-2) mRNA genes expression. Interestingly, co-administration with garlic or allopurinol improved the lipid peroxidation, antioxidant disturbance, and apoptosis induced by fipronil in the brain tissues. In conclusion, garlic or allopurinol reduced fipronil-induced apoptosis and reduced oxidative tissue damage, most likely through enhancing the tissue antioxidant defense system.
氟虫腈可导致组织氧化损伤和细胞凋亡。我们的目标是评估大蒜或别嘌呤醇对氟虫腈神经毒性的抗凋亡作用。将36只成年雄性白化大鼠分为对照组、大蒜水提取物组（500毫克/千克）、别嘌呤醇组（饮用水中含150毫克/升）、氟虫腈组（13.277毫克/千克）、大蒜+氟虫腈组和别嘌呤醇+氟虫腈组。我们的结果显示，与对照组相比，氟虫腈使脑丙二醛、蛋白质羰基水平以及酶促抗氧化活性（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和黄嘌呤氧化酶）显著升高，但谷胱甘肽-S-转移酶显著降低。此外，氟虫腈显著上调脑促凋亡蛋白（Bax）和半胱天冬酶-3 mRNA基因表达并诱导DNA片段化，但导致抗凋亡蛋白（Bcl-2）mRNA基因表达下调。有趣的是，与大蒜或别嘌呤醇共同给药可改善氟虫腈诱导的脑组织脂质过氧化、抗氧化紊乱和细胞凋亡。总之，大蒜或别嘌呤醇可减少氟虫腈诱导的细胞凋亡并减轻组织氧化损伤，很可能是通过增强组织抗氧化防御系统实现的。
