The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, P.R. China.
School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, P.R. China.
Analyst. 2023 Apr 11;148(8):1694-1702. doi: 10.1039/d3an00190c.
Herein, a competitive-type electrochemiluminescence immunosensor for ultrasensitive detection of 5-fluorouracil (5-FU) was fabricated. Ruthenium(II)-metal-organic framework (Ru-MOF) nanosheets were selected to act a promising ECL luminophore using tris(4,4'-dicarboxylic acid-2,2'-bipyridyl) ruthenium(II) dichloride (Ru(dcbpy)) as the organic ligand. The two-dimensional (2D) Ru-MOF nanosheets achieved an increased loading of Ru(dcbpy) and effectively prevented leakage of the ECL emitter during application, which exhibited satisfactory ECL performance. Thin two-dimensional MoS@GO was used to modify the electrode as the sensing platform for improving the electron transfer rate and loading more 5-FU coating antigens due to its large specific surface area and piezoelectric catalytic efficiency. Under the optimized conditions, the proposed immunosensor presented high sensitivity, a wide detection range (0.0001 ng-100 ng mL), a low limit of detection (0.031 pg mL, S/N = 3), good specificity and stability. Furthermore, the immunosensor was successfully applied for the detection of 5-FU in human serum samples with satisfactory results, proving this strategy has potential applications in bioanalysis and clinical diagnosis.
本文构建了一种用于超灵敏检测 5-氟尿嘧啶（5-FU）的竞争型电化学发光免疫传感器。选用钌（II）-金属有机骨架（Ru-MOF）纳米片作为有前途的电致化学发光（ECL）发光体，使用三（4,4'-二羧酸-2,2'-联吡啶）钌（II）二氯化物（Ru(dcbpy))作为有机配体。二维（2D）Ru-MOF 纳米片实现了 Ru(dcbpy)的高负载量，并在应用过程中有效防止了 ECL 发射器的泄漏，表现出令人满意的 ECL 性能。薄二维 MoS@GO 被用于修饰电极作为传感平台，由于其大的比表面积和压电催化效率，提高了电子转移率和负载更多的 5-FU 涂层抗原。在优化条件下，所提出的免疫传感器表现出高灵敏度、宽检测范围（0.0001 ng-100 ng mL）、低检测限（0.031 pg mL，S/N = 3）、良好的特异性和稳定性。此外，该免疫传感器成功应用于人血清样品中 5-FU 的检测，结果令人满意，证明该策略在生物分析和临床诊断中有潜在的应用。
